Mouse FAP Gene cDNA Clone / ORF Clone

FAP cDNA Clone Datasheet

FAP cDNA Clone Product Information

• Gene Bank Ref.ID: NM_007986.2
• cDNA Size: 2286
• Gene Synonym: Fap
• Source: Mouse

FAP cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus fibroblast activation protein DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

FAP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FAP cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FAP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGACAT GGCTGAAAAC TGTCTTTGGA GTTACCACCC TGGCTGCGCT TGCTTTAGTG
61       GTGATATGCA TTGTCTTACG TCCCTCAAGA GTTTACAAAC CTGAAGGAAA CACAAAGAGA
121      GCTCTTACCT TGAAGGATAT TTTAAATGGA ACATTCTCAT ATAAAACATA TTTTCCCAAC
181      TGGATTTCAG AACAAGAATA TCTTCATCAA TCTGAGGATG ATAACATAGT ATTTTATAAT
241      ATTGAAACAA GAGAATCATA TATCATTTTG AGTAATAGCA CCATGAAAAG TGTGAATGCT
301      ACAGATTATG GTTTGTCACC TGATCGGCAA TTTGTGTATC TAGAAAGTGA TTATTCAAAG
361      CTCTGGCGAT ATTCATACAC AGCGACATAC TACATCTACG ACCTTCAGAA TGGGGAATTT
421      GTAAGAGGAT ACGAGCTCCC TCGTCCAATT CAGTATCTAT GCTGGTCGCC TGTTGGGAGT
481      AAATTAGCAT ATGTATATCA AAACAATATT TATTTGAAAC AAAGACCAGG AGATCCACCT
541      TTTCAAATAA CTTATACTGG AAGAGAAAAT AGAATATTTA ATGGAATACC AGACTGGGTT
601      TATGAAGAGG AAATGCTTGC CACAAAATAT GCTCTTTGGT GGTCTCCAGA TGGAAAATTT
661      TTGGCATATG TAGAATTTAA TGATTCAGAT ATACCAATTA TTGCCTATTC TTATTATGGT
721      GATGGACAGT ATCCTAGAAC TATAAATATT CCATATCCAA AGGCTGGGGC TAAGAATCCG
781      GTTGTTCGTG TTTTTATTGT TGACACCACC TACCCTCACC ACGTGGGCCC AATGGAAGTG
841      CCAGTTCCAG AAATGATAGC CTCAAGTGAC TATTATTTCA GCTGGCTCAC ATGGGTGTCC
901      AGTGAACGAG TATGCTTGCA GTGGCTAAAA AGAGTGCAGA ATGTCTCAGT CCTGTCTATA
961      TGTGATTTCA GGGAAGACTG GCATGCATGG GAATGTCCAA AGAACCAGGA GCATGTAGAA
1021     GAAAGCAGAA CAGGATGGGC TGGTGGATTC TTTGTTTCGA CACCAGCTTT TAGCCAGGAT
1081     GCCACTTCTT ACTACAAAAT ATTTAGCGAC AAGGATGGTT ACAAACATAT TCACTACATC
1141     AAAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATATATATA
1201     TTCCGCGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG TTACCCTGGA
1261     AGAAGAAACA TCTACAGAAT TAGCATTGGA AACTCTCCTC CGAGCAAGAA GTGTGTTACT
1321     TGCCATCTAA GGAAAGAAAG GTGCCAATAT TACACAGCAA GTTTCAGCTA CAAAGCCAAG
1381     TACTATGCAC TCGTCTGCTA TGGCCCTGGC CTCCCCATTT CCACCCTCCA TGATGGCCGC
1441     ACAGACCAAG AAATACAAGT ATTAGAAGAA AACAAAGAAC TGGAAAATTC TCTGAGAAAT
1501     ATCCAGCTGC CTAAAGTGGA GATTAAGAAG CTCAAAGACG GGGGACTGAC TTTCTGGTAC
1561     AAGATGATTC TGCCTCCTCA GTTTGACAGA TCAAAGAAGT ACCCTTTGCT AATTCAAGTG
1621     TATGGTGGTC CTTGCAGCCA GAGTGTTAAG TCTGTGTTTG CTGTTAATTG GATAACTTAT
1681     CTCGCAAGTA AGGAGGGGAT AGTCATTGCC CTGGTAGATG GTCGGGGCAC TGCTTTCCAA
1741     GGTGACAAAT TCCTGCATGC CGTGTATCGA AAACTGGGTG TATATGAAGT TGAGGACCAG
1801     CTCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAGAAAG AATAGCCATA
1861     TGGGGCTGGT CCTACGGAGG TTATGTTTCA TCCCTGGCCC TTGCATCTGG AACTGGTCTT
1921     TTCAAATGTG GCATAGCAGT GGCTCCAGTC TCCAGCTGGG AATATTACGC ATCTATCTAC
1981     TCAGAGAGAT TCATGGGCCT CCCAACAAAG GACGACAATC TCGAACACTA TAAAAATTCA
2041     ACTGTGATGG CAAGAGCAGA ATATTTCAGA AATGTAGACT ATCTTCTCAT CCACGGAACA
2101     GCAGATGATA ATGTGCACTT TCAGAACTCA GCACAGATTG CTAAAGCTTT GGTTAATGCA
2161     CAAGTGGATT TCCAGGCGAT GTGGTACTCT GACCAGAACC ATGGTATATC ATCTGGGCGC
2221     TCCCAGAATC ATTTATATAC CCACATGACG CACTTCCTCA AGCAATGCTT TTCTTTATCA
2281     GACTGA

 

Quality Control:cDNA sequencing results is available upon request.