Mouse Fgf8 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
MG51101-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

Fgf8 cDNA Clone Datasheet

Fgf8 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001166361.1
cDNA Size:	732
Gene Synonym:	Fgf8
Source:	Mouse

Fgf8 cDNA Clone Description

cDNA Description:	ORF Clone of Mus musculus fibroblast growth factor 8 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Same as Gene Bank Ref. ID sequence with the nucleotide 733-735 TAG deletion mutation.

Fgf8 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Fgf8 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

Fgf8 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCAGCC CCCGCTCCGC GCTGAGCTGC CTGCTGTTGC ACTTGCTGGT TCTCTGCCTC
61       CAAGCCCAGG AAGGCCCGGG CGGGGGGCCT GCGCTGGGCA GGGAGCCCAC TTCCCTGCTC
121      CGAGCTGGCC GGGAGCCCCA GGGTGTTTCC CAACAGGTAA CTGTTCAGTC CTCACCTAAT
181      TTTACACAGC ATGTGAGGGA GCAGAGCCTG GTGACGGATC AGCTCAGCCG CCGCCTCATC
241      CGGACCTACC AGCTCTACAG CCGCACCAGC GGGAAGCACG TGCAGGTCCT GGCCAACAAG
301      CGCATCAACG CCATGGCAGA AGACGGAGAC CCCTTCGCGA AGCTCATTGT GGAGACCGAT
361      ACTTTTGGAA GCAGAGTCCG AGTTCGCGGC GCAGAGACAG GTCTCTACAT CTGCATGAAC
421      AAGAAGGGGA AGCTAATTGC CAAGAGCAAC GGCAAAGGCA AGGACTGCGT ATTCACAGAG
481      ATCGTGCTGG AGAACAACTA CACGGCGCTG CAGAACGCCA AGTACGAGGG CTGGTACATG
541      GCCTTTACCC GCAAGGGCCG GCCCCGCAAG GGCTCCAAGA CGCGCCAGCA TCAGCGCGAG
601      GTGCACTTCA TGAAGCGCCT GCCGCGGGGC CACCACACCA CCGAGCAGAG CCTGCGCTTC
661      GAGTTCCTCA ACTACCCGCC CTTCACGCGC AGCCTGCGCG GCAGCCAGAG GACTTGGGCC
721      CCGGAGCCCC GA

Quality Control:cDNA sequencing results is available upon request.