|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FetuinA / AHSG ORF mammalian expression plasmid, C-GFPSpark tag||HG10318-ACG|
|Human FetuinA / AHSG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10318-ACR|
|Human FetuinA / AHSG ORF mammalian expression plasmid, C-Flag tag||HG10318-CF|
|Human FetuinA / AHSG ORF mammalian expression plasmid, C-His tag||HG10318-CH|
|Human FetuinA / AHSG ORF mammalian expression plasmid, C-Myc tag||HG10318-CM|
|Human FetuinA / AHSG ORF mammalian expression plasmid, C-HA tag||HG10318-CY|
|Human FetuinA / AHSG Gene cDNA clone plasmid||HG10318-M|
|Human FetuinA / AHSG ORF mammalian expression plasmid, N-Flag tag||HG10318-NF|
|Human FetuinA / AHSG ORF mammalian expression plasmid, N-His tag||HG10318-NH|
|Human FetuinA / AHSG ORF mammalian expression plasmid, N-Myc tag||HG10318-NM|
|Human FetuinA / AHSG ORF mammalian expression plasmid, N-HA tag||HG10318-NY|
|Human FetuinA / AHSG natural ORF mammalian expression plasmid||HG10318-UT|
|Learn more about expression Vectors|
Fetuin-A, also known as Alpha-2-HS-Glycoprotein (AHSG), belongs to the Fetuin family, is a plasma binding protein, and is more abundant in fetal than adult blood. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Thus Fetuin-A is a potent inhibitor of pathological calcification. The circulating levels of fetuin-A, a well-described inhibitor of calcification, regulate the cell-dependent process of osteogenesis. The low circulating fetuin-A levels are associated with a greater prevalence and/or severity of Vascular calcification (VC) and increased risk for all-cause and cardiovascular mortality. However, high circulating fetuin-A levels appear to induce insulin resistance and, in non-dialyzed subjects with diabetic nephropathy, are directly related to VC burden. The emerging role of fetuin-A deficiency as a risk factor in dialysis patients was documented in cross-sectional studies demonstrating a significant correlation with all-cause and cardiovascular mortality. Additionally, Human fetuin-A is a negative acute phase protein involved in inflammatory diseases, thus being a potential physiological regulator of meprin activity. Fetuin-A is a broad-range protease inhibitor. Fetuin-A and cystatin C as endogenous proteolytic regulators of meprin activity broadens our understanding of the proteolytic network in plasma.