Fetuin-B / FETUB Antibody (Antigen Affinity Purified)

Fetuin-B / FETUB Antibody ( Antigen Affinity Purified ) Datasheet

Fetuin-B / FETUB Antibody Product Information

• Immunogen: Recombinant Human Fetuin-B / FETUB protein (Catalog#11834-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human Fetuin-B / FETUB (rh Fetuin-B / FETUB; Catalog#11834-H08H; NP_055190.2; Met 1-Pro 382). Fetuin-B / FETUB specific IgG was purified by Human Fetuin-B / FETUB affinity chromatography.

Fetuin-B / FETUB Antibody Usage Guide

• Specificity: Human Fetuin-B / FETUB
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human FETUB in WB. Using a DAB detection system, the detection limit for Human FETUB is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human FETUB. The detection limit for Human FETUB is approximately 0.00975 ng/well.6
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Fetuin-B / FETUB Antibody Background

Fetuin-B, also known as Fetuin-like protein IRL685 and FETUB, is a secreted protein which belongs to thefetuin family. Fetuin-B / FETUB contains twocystatin domains. Fetuin-B is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. Fetuin-A has been identified as a major protein during fetal life and is also involved in important functions such as inhibition of the insulin receptor tyrosine kinase activity, protease inhibitory activities and development-associated regulation of calcium metabolism and osteogenesis. Fetuin-A is a key partner in the recovery phase of an acute inflammatory response. Fetuin-B / FETUB is found at least in human and rodents. It is unambiguously a paralogue of Fetuin-A. Fetuin-A and Fetuin-B exhibit significant differences at the amino acid sequence level, notably including variations with respect to the archetypal fetuin-specific signature.

References

1. Olivier E., et al., 2000, Biochem. J. 350:589-97.
2. Liu T., et al., 2005, J. Proteome Res. 4:2070-80.
3. Hsu SJ, et al., 2005, Genome. 47 (5): 931-46.
4. Liu T, et al., 2006, J. Proteome Res.4 (6): 2070-80.