PRMT6 cDNA ORF Clone, Ferret, N-HA tag

Cat: FG60131-NY
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All PRMT6 Reagents
CRO Services
Product recommended

All PRMT6 Reagents
PRMT6 cDNA ORF Clone, Ferret, N-HA tag General Information
Gene
Species
Ferret
NCBI Ref Seq
RefSeq ORF Size
1128 bp
Description
Full length Clone DNA of Ferret protein arginine methyltransferase 6 with N terminal HA tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-N-HA
Tag Sequence
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

PRMT6 Background Information

Protein arginine N-methyltransferase 6, also known as Histone-arginine N-methyltransferase PRMT6, PRMT6, and HRMT1L6, is a member of the protein arginine N-methyltransferase family and PRMT6 subfamily. PRMT6 is highly expressed in kidney and testes. PRMT6 is known to catalyze the generation of asymmetric dimethylarginine in polypeptides. It has been implicated in human immunodeficiency virus pathogenesis, DNA repair, and transcriptional regulation. PRMT6 is known to methylate histone H3 Arg-2 (H3R2), and this negatively regulates the lysine methylation of H3K4 resulting in gene repression. PRMT6 plays a key role in coupling process by functioning as a transcriptional coactivator that can regulate alternative splicing. PRMT6 coactivates the progesterone, glucocorticoid and oestrogen receptors in luciferase reporter assays in a hormone-dependent manner. Small interfering RNA (siRNA) oligonucleotide duplex knockdown of PRMT6 disrupts oestrogen-stimulated transcription of endogenous GREB1 and progesterone receptor in MCF-7 breast cancer cells. Neutralizing the activity of PRMT6 could inhibit tumor progression and may be of cancer therapeutic significance.

Full Name
protein arginine methyltransferase 6
References
  • Hyllus D, et al., 2007, Genes Dev. 21(24): 3369-80.
  • Lakowski, TM. et al., 2008, J Biol Chem. 283 (15): 10015-25. 
  • Michaud-Levesque, J. et al., 2009, J Biol Chem. 284 (32): 21338-46.
  • Harrison, MJ. et al., 2010, Nucleic acids Res. Jan 4.
  • PRMT6 cDNA ORF Clone, Ferret, Related Products

    Product Name Catalog#(PDF)
    PRMT6 cDNA ORF Clone in Cloning Vector, Ferret FG60131-G
    PRMT6 cDNA ORF Clone, Ferret, untagged FG60131-UT
    PRMT6 Lentiviral cDNA ORF Clone, Ferret, C-GFPSpark® tag FG60131-ACGLN
    PRMT6 cDNA ORF Clone, Ferret, C-GFPSpark® tag FG60131-ACG
    PRMT6 cDNA ORF Clone, Ferret, N-GFPSpark® tag FG60131-ANG
    PRMT6 cDNA ORF Clone, Ferret, C-OFPSpark® tag FG60131-ACR
    PRMT6 cDNA ORF Clone, Ferret, N-OFPSpark® tag FG60131-ANR
    PRMT6 cDNA ORF Clone, Ferret, C-DDK (Flag®) tag FG60131-CF
    PRMT6 cDNA ORF Clone, Ferret, N-DDK (Flag®) tag FG60131-NF
    PRMT6 cDNA ORF Clone, Ferret, C-His tag FG60131-CH
    PRMT6 cDNA ORF Clone, Ferret, N-His tag FG60131-NH
    PRMT6 cDNA ORF Clone, Ferret, C-HA tag FG60131-CY
    PRMT6 cDNA ORF Clone, Ferret, C-Myc tag FG60131-CM
    PRMT6 cDNA ORF Clone, Ferret, N-Myc tag FG60131-NM

    PRMT6 cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    PRMT6 cDNA ORF Clone in Cloning Vector, Mouse MG51052-G
    PRMT6 cDNA ORF Clone, Mouse, untagged MG51052-UT
    PRMT6 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51052-ACGLN
    PRMT6 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51052-ACG
    PRMT6 cDNA ORF Clone, Mouse, N-GFPSpark® tag MG51052-ANG
    PRMT6 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51052-ACR
    PRMT6 cDNA ORF Clone, Mouse, N-OFPSpark® tag MG51052-ANR
    PRMT6 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51052-CF
    PRMT6 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG51052-NF
    PRMT6 cDNA ORF Clone, Mouse, C-His tag MG51052-CH
    PRMT6 cDNA ORF Clone, Mouse, N-His tag MG51052-NH
    PRMT6 cDNA ORF Clone, Mouse, C-HA tag MG51052-CY
    PRMT6 cDNA ORF Clone, Mouse, N-HA tag MG51052-NY
    PRMT6 cDNA ORF Clone, Mouse, C-Myc tag MG51052-CM
    PRMT6 cDNA ORF Clone, Mouse, N-Myc tag MG51052-NM
    PRMT6 qPCR Primer Pairs, Mouse MP201010

    PRMT6 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    PRMT6 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11313-ACGLN
    PRMT6 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11313-CF
    PRMT6 cDNA ORF Clone, Human, C-His tag HG11313-CH
    PRMT6 qPCR Primer Pairs, Human HP101277
    info info
    添加购物车成功! 添加购物车失败!