|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Ferret CAT ORF mammalian expression plasmid, C-GFPSpark tag||FG60113-ACG|
|Ferret CAT ORF mammalian expression plasmid, C-OFPSpark / RFP tag||FG60113-ACR|
|Ferret CAT ORF mammalian expression plasmid, C-Flag tag||FG60113-CF|
|Ferret CAT ORF mammalian expression plasmid, C-His tag||FG60113-CH|
|Ferret CAT ORF mammalian expression plasmid, C-Myc tag||FG60113-CM|
|Ferret CAT ORF mammalian expression plasmid, C-HA tag||FG60113-CY|
|Ferret CAT Gene cDNA clone plasmid||FG60113-G|
|Ferret CAT ORF mammalian expression plasmid, N-Flag tag||FG60113-NF|
|Ferret CAT ORF mammalian expression plasmid, N-His tag||FG60113-NH|
|Ferret CAT ORF mammalian expression plasmid, N-Myc tag||FG60113-NM|
|Ferret CAT ORF mammalian expression plasmid, N-HA tag||FG60113-NY|
|Ferret CAT natural ORF mammalian expression plasmid||FG60113-UT|
|Learn more about expression Vectors|
Catalase is a ubiquitously expressed enzyme that catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a tetramer of four polypeptides chains containing four porphyrin heme groups that allow the enzyme to react with the hydrogen peroxide. The optimum PH of human catalase is approximately 7 and the optimum temperature is at 37 degree. Both the PH optimum and temperature for other catalases varies depending on the species. Catalase can be inhibited by a flux of O2- generated in situ by the aerobic xanthine oxidase reaction. This inhibition of catalase by O2- provides the basis for a synergism between superoxide dismutase and catalase.Such synergisms have been observed in vitro and may be significant in vivo. Catalase is used in the food industry for removing hydrogen peroxide from milk prior to cheese production. Another use is in food wrappers where it prevents food from oxidizing. Catalase is also used in the textile industry, removing hydrogen peroxide from fabrics to make sure the material is peroxide-free.