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Rat FZD9 Gene cDNA Clone (full-length ORF Clone)
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Rat FZD9 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
RG80388-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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FZD9 cDNA Clone Datasheet

 

FZD9 cDNA Clone Product Information

Gene Bank Ref.ID: NM_153305.1

cDNA Size:

1779

Gene Synonym:

Fzd9

Source: Rat

FZD9 cDNA Clone Description

cDNA Description:

ORF Clone of Rattus norvegicus frizzled homolog 9 (Drosophila) DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1260 A/C and 1458 C/T not causing the amino acid variation.

FZD9 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FZD9 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FZD9 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGGTGC CACCGCTGCT CCGCGGGGCG CTGCTGCTGT GGCAGCTGCT GGCGACGGGC
61       GGCGCGGCGC TGGAGATCGG CCGCTTTGAT CCGGAGCGCG GCCGTGGCCC CGCACCGTGC
121      CAAGCGATGG AGATCCCCAT GTGCCGGGGC ATCGGCTACA ACCTGACCCG CATGCCCAAC
181      CTACTGGGCC ACACGTCGCA GGGCGAGGCG GCCGCGCAGC TGGCCGAGTT CTCGCCTCTC
241      GTGCAGTACG GCTGCCACAG CCACCTGCGC TTCTTCCTCT GCTCGCTCTA CGCCCCAATG
301      TGCACCGACC AGGTCTCCAC TCCCATCCCC GCCTGCCGGC CCATGTGCGA GCAGGCTCGC
361      CTGCGCTGCG CCCCCATCAT GGAGCAATTC AATTTCGGCT GGCCGGACTC CCTCGACTGT
421      GCCCGGCTCC CCACGCGCAA CGACCCGCAC GCGCTCTGCA TGGAGGCACC CGAGAACGCT
481      ACCGCAGGCC CCACAGAACC CCACAAGGGC CTGGGCATGC TTCCTGTAGC ACCTCGGCCC
541      GCGAGGCCAC CGGGAGATTC AGCCCCCGGT CCGGGCAGTG GAGGCACCTG CGACAACCCC
601      GAGAAGTTCC AGTACGTGGA GAAGAGTCGC TCGTGCGCTC CGCGCTGCGG GCCAGGCGTC
661      GAGGTGTTCT GGTCTCGGCG CGATAAGGAC TTCGCGCTGG TCTGGATGGC TGTGTGGTCC
721      GCGTTGTGTT TCTTCTCCAC GGCCTTCACC GTGTTCACCT TCCTGCTGGA GCCTCACCGA
781      TTCCAGTACC CAGAGCGCCC TATAATCTTC CTTTCCATGT GCTACAATGT CTACTCCTTG
841      GCCTTCCTGA TCAGAGCGGT GGCAGGTGCA CAGAGTGTGG CCTGCGACCA GGAGGCAGGG
901      GCTCTGTATG TGATCCAGGA GGGTTTGGAA AACACAGGCT GCACCCTGGT CTTCCTATTG
961      CTCTATTATT TCGGGATGGC CAGCTCACTT TGGTGGGTGG TTTTGACTCT CACCTGGTTC
1021     CTGGCTGCAG GCAAAAAGTG GGGCCACGAA GCCATCGAGG CTCACGGTAG TTACTTCCAC
1081     ATGGCAGCGT GGGGCTTGCC AGCACTCAAG ACTATCGTGG TCCTGACTCT ACGTAAGGTG
1141     GCTGGGGATG AGCTGACTGG GCTCTGCTAT GTAGCTAGCA TGGACCCGGC AGCTCTCACT
1201     GGCTTTGTGT TGGTGCCCCT CTCTTGCTAC TTGGTACTGG GCACCAGTTT CCTCCTGACC
1261     GGTTTTGTGG CTCTCTTCCA TATCCGCAAA ATTATGAAGA CGGGCGGCAC CAATACAGAG
1321     AAGCTGGAGA AGCTGATGGT GAAAATCGGA GTCTTTTCCA TCCTTTACAC AGTGCCGGCT
1381     ACCTGCGTCA TTGTCTGCTA TGTTTACGAA CGCCTCAACA TGGACTTCTG GCGTCTTCGG
1441     GCCACAGAGC AACCATGTAC TGCCGCTGCC GTGCCTGGAG GCCGGAGAGA CTGCTCGCTG
1501     CCAGGGGGCT CGGTGCCCAC TGTGGCCGTC TTCATGCTCA AAATCTTCAT GTCCTTGGTG
1561     GTGGGCATCA CCAGTGGAGT CTGGGTATGG AGTTCCAAGA CTTTCCAGAC TTGGCAGAGT
1621     CTATGTTACC GAAAAATGGC AGCTGGTCGA GCCCGGGCCA AGGCCTGCCG AACCCCAGGG
1681     GGCTATGGCC GGGGTACCCA CTGTCACTAC AAAGCCCCCA CGGTGGTCTT GCACATGACT
1741     AAGACAGACC CCTCTCTGGA GAACCCCACA CACCTCTAG

 

Quality Control:cDNA sequencing results is available upon request.

 
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"