Rat FZD4 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80377-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

FZD4 cDNA Clone Datasheet

FZD4 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_022623.1
cDNA Size:	1617
Gene Synonym:	Fzd4
Source:	Rat

FZD4 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus frizzled homolog 4 (Drosophila) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

FZD4 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FZD4 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FZD4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTTGGC AGGGCACAGG GCCAAGCGTC CGGGGGATGC CTGGAGGCGT CAGGCTCAGG
61       CTGGGGCTGC TGCTGCTGCA GTTGCTCCTG CTCCAGCGGC CCGCCCTGGG GTTCGGGGAC
121      GAGGAGGAGC GGCGTTGCGA CCCCATCCGC ATCGCCATGT GCCAGAACCT CGGCTACAAC
181      GTGACCAAGA TGCCCAACTT AGTGGGACAC GAGCTGCAGA CAGACGCGGA GCTGCAGCTG
241      ACAACTTTCA CGCCGCTCAT CCAGTACGGC TGCTCCAGCC AGCTGCAGTT CTTCCTTTGT
301      TCGGTTTATG TGCCAATGTG CACAGAGAAG ATCAACATCC CCATCGGCCC GTGCGGTGGC
361      ATGTGTCTTT CAGTCAAGAG ACGATGTGAA CCAGTCCTGA AAGAATTTGG GTTTGCCTGG
421      CCGGACAGCC TGAACTGCAG CAAGTTCCCA CCCCAGAACG ACCACAACCA CATGTGCATG
481      GAAGGACCAG GTGACGAAGA GGTACCCTTG CCCCACAAAA CTCCCATCCA GCCGGGGGAA
541      GAGTGCCACT CCGTGGGAAC CAATTCCGAT CAGTACATCT GGGTGAAAAG GAGCCTGAAC
601      TGTGTTCTCA AGTGTGGCTA CGATGCTGGC TTGTACAGCC GCTCAGCTAA GGAGTTCACG
661      GATATTTGGA TGGCCGTGTG GGCCAGCCTC TGCTTCATCT CTACCACCTT CACTGTGCTG
721      ACCTTCCTGA TTGATTCGTC CAGGTTTTCT TACCCTGAGC GCCCCATCAT ATTCCTCAGT
781      ATGTGCTATA ATATTTATAG CATTGCTTAT ATTGTTCGGC TTACTGTAGG CCGGGAAAGG
841      ATATCCTGTG ATTTTGAAGA GGCGGCAGAA CCCGTTCTCA TCCAAGAGGG ACTTAAGAAC
901      ACAGGATGTG CAATAATTTT CTTGCTGATG TACTTTTTTG GAATGGCCAG CTCCATTTGG
961      TGGGTTATTC TGACACTCAC TTGGTTTTTG GCAGCCGGAC TCAAGTGGGG TCACGAAGCC
1021     ATTGAAATGC ACAGTTCTTA TTTCCACATC GCAGCCTGGG CTATCCCTGC CGTGAAAACC
1081     ATTGTCATCT TGATTATGAG ACTAGTGGAT GCCGATGAGC TGACTGGCCT GTGCTATGTC
1141     GGGAACCAAA GCCTAGATGC CCTCACCGGC TTTGTGGTGG CACCTCTCTT TACCTATTTG
1201     GTGATTGGAA CTCTATTCAT TGCTGCAGGC TTGGTGGCCC TCTTCAAAAT TCGGTCAAAT
1261     CTTCAAAAAG ATGGGACCAA GACAGACAAG TTGGAAAGGC TAATGGTCAA GATCGGGGTC
1321     TTCTCGGTCC TGTACACGGT TCCTGCGACC TGTGTGATTG CCTGTTATTT CTATGAAATC
1381     TCAAACTGGG CACTCTTTCG GTATTCTGCA GATGACTCAA ACATGGCAGT TGAAATGTTG
1441     AAAATTTTTA TGTCTTTGCT CGTGGGCATC ACTTCAGGCA TGTGGATTTG GTCTGCCAAA
1501     ACTCTTCACA CGTGGCAAAA ATGTTCTAAC CGATTGGTGA ATTCTGGGAA GGTAAAGAGA
1561     GAGAAGAGGG GGAATGGTTG GGTGAAGCCA GGGAAAGGCA ACGAAACTGT GGTGTAG

Quality Control:cDNA sequencing results is available upon request.