Mouse FVII / Factor VII / F7 Protein (His Tag)

Coagulation factor VII Protein Datasheet

FVII / Factor VII / F7 Protein Product Information

• Synonym: AI132620, Cf7, FVII, mfVII, F7
• Protein Construction: A DNA sequence encoding the mouse FⅦ (NP_034302.2) (Met 1-Leu 446) was fused with the a polyhistidine tag at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

FVII / Factor VII / F7 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 25 & Ala 42
• Molecular Mass: The mature form of mouse FⅦ consists of 416 amino acids after removal of the signal peptide and the propeptid, and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FⅦ is approximately 56-63 kDa due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FVII / Factor VII / F7 protein

FVII / Factor VII / F7 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FVII / Factor VII / F7 Protein Description

Coagulation factor VII, also known as Serum prothrombin conversion accelerator, Factor VII, F7 and FVII, is a member of the peptidase S1 family. Factor VII is one of the central proteins in the coagulation cascade. It is an enzyme of the serine protease class. Factor VII contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one peptidase S1 domain. The main role of factor VII is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels, normally not exposed to the blood stream. The action of the Factor VII is impeded by tissue factor pathway inhibitor (TFPI), which is released almost immediately after initiation of coagulation. Factor VII is vitamin K dependent and is produced in the liver. The use of warfarin or similar anticoagulants impairs its function. Upon vessel injury, tissue factor is exposed to the blood and circulating Factor VII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. The most important substrates for FVIIa-TF are Factor X and Factor IX.

References

1. Kenet G. et al., 1999, Lancet. 354?(9193): 1879.
2. Roberts H. et al., 2004, Blood. 104?(13): 3858-64.
3. Mayer S. et al., 2005, N Engl J Med.?352?(8): 777-85.
4. O'Connell K. et al., 2006, JAMA. 295 (3): 293-8.