FUT8 (aa 68-575) Protein (His Tag)

Fucosyltransferase 8 (alpha (1,6) fucosyltransferase) Protein Datasheet

FUT8 (aa 68-575) Protein Product Information

• Synonym: MGC26465
• Protein Construction: A DNA sequence encoding the human FUT8 isoform 1 (Q9BYC5-1) (Arg 68-Lys 575) was fused with a polyhistidine tag at the carboxy-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

FUT8 (aa 68-575) Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Arg 68
• Molecular Mass: The recombinant human FUT8 consists of 518 amino acids and has a calculated molecular mass of 60 KDa. It migrates as an approximately 55 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FUT8 (aa 68-575) protein

FUT8 (aa 68-575) Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FUT8 (aa 68-575) Protein Description

Human alpha (1,6) fucosyltransferase 8, also known as FUT8, is a member of the?glycosyltransferase family. Fucosyltransferases are the enzymes transferring fucose from GDP-Fuc to Gal in an alpha1,2-linkage and to GlcNAc in alpha1,3-linkage, alpha1,4-linkage, or alpha1,6-linkage. All fucosyltransferases utilize the same nucleotide sugar, their specificity reside in the recognition of the acceptor and in the type of linkage formed. Fucosyltransferases share some common structural and catalytic features. On the basis of protein sequence similarities, these enzymes can be classified into four distinct families: (1) the alpha-2-fucosyltransferases, (2) the alpha-3-fucosyltransferases, (3) the mammalian alpha-6-fucosyltransferases, and (4) the bacterial alpha-6-fucosyltransferases. The alpha-3-fucosyltransferases constitute a distinct family as they lack the consensus peptide, but some regions display similarities with the alpha-2 and alpha-6-fucosyltranferases.

References

1. Breton C. et al., 1998, Glycobiology. 8 (1): 87-94.
2. Oriol R. et al., 1999, Glycobiology. 9 (4): 323-34.
3. De Vries T. et al., 2001, Glycobiology. 11 (10): 119-28.
4. Baboval T. et al., 2002, Mamm Genome. 13: 538-41.