FTLS / RSPO2 Protein

R-spondin 2 Protein Datasheet

FTLS / RSPO2 Protein Product Information

• Synonym: UNQ9384, PRO34209, CRISTIN2, MGC35555, MGC43342
• Protein Construction: A DNA sequence encoding the human RSPO2 (Q6UXX9-1) N-teminal fragment (Met 1- Gly 205, 186 Leu/Pro) was fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Human
• Expression Host: Human Cells

FTLS / RSPO2 Protein QC Testing

• Purity: >90 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 22
• Molecular Mass: The secreted recombinant human RSPO2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 425 amino acids and has a predicted molecular mass of 48.3 kDa. ?The apparent molecular mass of rh RSPO2/Fc monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FTLS / RSPO2 protein

FTLS / RSPO2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FTLS / RSPO2 Protein Description

R-spondin-2, also known as RSPO2, synergizes with Wnt to activate beta-catenin. RSPO2 is secreted proteins that regulate beta-catenin signaling. Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt / beta- catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled / LRP receptors. Probably also acts as a ligand for frizzled and LRP receptors. The encoding gene Rspo2 was identified as a novel common integration site for the mouse mammary tumor virus in viral induced mouse mammary tumors. Rspo2 and Rspo2 / Wnt1 tumors contained many spindle cells, consistent with an epithelial-mesenchymal transformation phenotype. When Rspo2 and Rspo2 / Wnt1 tumor cells were transferred into na?ve mice, they exhibited greater metastatic activity than cells derived from Wnt1 tumors.

References

1. Cadieu E. et al., 2009, Science. 326: 150-3.
2. Kazanskaya O. et al., 2004, Dev Cell. 7 (4): 525-34.
3. Parker HG. et al., 2010, J Hered. 101 (5): 612-7.