FTH1 Protein (Native)

Ferritin, heavy polypeptide 1 Protein Datasheet

FTH1 Protein Product Information

• Synonym: OK/SW-cl.84, FHC, FTH, FTHL6, PIG15, PLIF
• Protein Construction: A DNA sequence encoding the human FTH1 (P02794) (Met 1-Ser183) was expressed and purified.
• Source: Human
• Expression Host: E.coli

FTH1 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human FTH1 consists of 183 amino acids and has a calculated molecular mass of 21.2KDa as estimated in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FTH1 protein

FTH1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FTH1 Protein Description

FTH1(ferritin, heavy polypeptide 1) is the heavy subunit of ferritin. Ferritin is a ubiquitous intracellular protein that stores iron and releases it in a controlled fashion. The protein is produced by almost all living organisms, including algae, bacteria and higher plants, and animals. In humans, it acts as a buffer against iron deficiency and iron overload. The amount of ferritin stored reflects the amount of iron stored. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Deficiency of ferritin proteins is associated with several neurodegenerative diseases. FTH1 stores iron in a soluble, non-toxic, readily available form. It is important for iron homeostasis. It has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. It also plays a role in delivery of iron to cells and mediates iron uptake in capsule cells of the developing kidney.

References

1. Hentze MW. et al., 1986, Proc Natl Acad Sci. 83 (19): 7226-30.
2. Rual. et al., 2005, Nature (England). 437 (7062): 1173-8.
3. Stelzl. et al., 2005, Cell (United States). 122 (6): 957-968.