FLT3L / Flt3 ligand / FLT3LG Protein (His Tag)

Fms-related tyrosine kinase 3 ligand Protein Datasheet

FLT3L / Flt3 ligand / FLT3LG Protein Product Information

• Synonym: FLT3LG
• Protein Construction: A DNA sequence encoding the human FLT3LG (P49771-1) (Thr27-Pro185) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

FLT3L / Flt3 ligand / FLT3LG Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant human human FLT3LG consists of 175 amino acids and predicts a molecular mass of 20.2 KDa. It migrates as an approximately 27 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris,500mM NaCl, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FLT3L / Flt3 ligand / FLT3LG protein

FLT3L / Flt3 ligand / FLT3LG Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FLT3L / Flt3 ligand / FLT3LG Protein Description

FLT3L, also known as flt3 ligand, acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It is a small molecule which also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3L is crucial for steady-state pDC and cDC development. It controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts.

References

1. Hannum C. et al., 1994, Nature. 368 (6472): 643-8.
2. Lyman SD. et al., 1995, Oncogene. 10 (1): 149-57.
3. Lyman SD. et al., 1994, Cell. 75 (6): 1157-67.