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> Gene Products > Human cDNA Clones > Human CD135 / FLK2 Gene cDNA Clone / ORF Clone Human CD135 / FLK2 Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| HG10445-M |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_004119.2
cDNA Size: 2982
Synonym: FLK2; STK1; CD135; FLT3
Source: Human.
cDNA Description: ORF Clone of Homo sapiens fms-related tyrosine kinase 3 DNA.
Vector:
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 680 C/T
resulting in the amino acid Thr substitution by Met and 288C/T not causing the amino acid variation.
5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:
1 ATGCCGGCGT TGGCGCGCGA CGGCGGCCAG CTGCCGCTGC TCGTTGTTTT TTCTGCAATG 61 ATATTTGGGA CTATTACAAA TCAAGATCTG CCTGTGATCA AGTGTGTTTT AATCAATCAT 121 AAGAACAATG ATTCATCAGT GGGGAAGTCA TCATCATATC CCATGGTATC AGAATCCCCG 181 GAAGACCTCG GGTGTGCGTT GAGACCCCAG AGCTCAGGGA CAGTGTACGA AGCTGCCGCT 241 GTGGAAGTGG ATGTATCTGC TTCCATCACA CTGCAAGTGC TGGTCGATGC CCCAGGGAAC 301 ATTTCCTGTC TCTGGGTCTT TAAGCACAGC TCCCTGAATT GCCAGCCACA TTTTGATTTA 361 CAAAACAGAG GAGTTGTTTC CATGGTCATT TTGAAAATGA CAGAAACCCA AGCTGGAGAA 421 TACCTACTTT TTATTCAGAG TGAAGCTACC AATTACACAA TATTGTTTAC AGTGAGTATA 481 AGAAATACCC TGCTTTACAC ATTAAGAAGA CCTTACTTTA GAAAAATGGA AAACCAGGAC 541 GCCCTGGTCT GCATATCTGA GAGCGTTCCA GAGCCGATCG TGGAATGGGT GCTTTGCGAT 601 TCACAGGGGG AAAGCTGTAA AGAAGAAAGT CCAGCTGTTG TTAAAAAGGA GGAAAAAGTG 661 CTTCATGAAT TATTTGGGAT GGACATAAGG TGCTGTGCCA GAAATGAACT GGGCAGGGAA 721 TGCACCAGGC TGTTCACAAT AGATCTAAAT CAAACTCCTC AGACCACATT GCCACAATTA 781 TTTCTTAAAG TAGGGGAACC CTTATGGATA AGGTGCAAAG CTGTTCATGT GAACCATGGA 841 TTCGGGCTCA CCTGGGAATT AGAAAACAAA GCACTCGAGG AGGGCAACTA CTTTGAGATG 901 AGTACCTATT CAACAAACAG AACTATGATA CGGATTCTGT TTGCTTTTGT ATCATCAGTG 961 GCAAGAAACG ACACCGGATA CTACACTTGT TCCTCTTCAA AGCATCCCAG TCAATCAGCT 1021 TTGGTTACCA TCGTAGAAAA GGGATTTATA AATGCTACCA ATTCAAGTGA AGATTATGAA 1081 ATTGACCAAT ATGAAGAGTT TTGTTTTTCT GTCAGGTTTA AAGCCTACCC ACAAATCAGA 1141 TGTACGTGGA CCTTCTCTCG AAAATCATTT CCTTGTGAGC AAAAGGGTCT TGATAACGGA 1201 TACAGCATAT CCAAGTTTTG CAATCATAAG CACCAGCCAG GAGAATATAT ATTCCATGCA 1261 GAAAATGATG ATGCCCAATT TACCAAAATG TTCACGCTGA ATATAAGAAG GAAACCTCAA 1321 GTGCTCGCAG AAGCATCGGC AAGTCAGGCG TCCTGTTTCT CGGATGGATA CCCATTACCA 1381 TCTTGGACCT GGAAGAAGTG TTCAGACAAG TCTCCCAACT GCACAGAAGA GATCACAGAA 1441 GGAGTCTGGA ATAGAAAGGC TAACAGAAAA GTGTTTGGAC AGTGGGTGTC GAGCAGTACT 1501 CTAAACATGA GTGAAGCCAT AAAAGGGTTC CTGGTCAAGT GCTGTGCATA CAATTCCCTT 1561 GGCACATCTT GTGAGACGAT CCTTTTAAAC TCTCCAGGCC CCTTCCCTTT CATCCAAGAC 1621 AACATCTCAT TCTATGCAAC AATTGGTGTT TGTCTCCTCT TCATTGTCGT TTTAACCCTG 1681 CTAATTTGTC ACAAGTACAA AAAGCAATTT AGGTATGAAA GCCAGCTACA GATGGTACAG 1741 GTGACCGGCT CCTCAGATAA TGAGTACTTC TACGTTGATT TCAGAGAATA TGAATATGAT 1801 CTCAAATGGG AGTTTCCAAG AGAAAATTTA GAGTTTGGGA AGGTACTAGG ATCAGGTGCT 1861 TTTGGAAAAG TGATGAACGC AACAGCTTAT GGAATTAGCA AAACAGGAGT CTCAATCCAG 1921 GTTGCCGTCA AAATGCTGAA AGAAAAAGCA GACAGCTCTG AAAGAGAGGC ACTCATGTCA 1981 GAACTCAAGA TGATGACCCA GCTGGGAAGC CACGAGAATA TTGTGAACCT GCTGGGGGCG 2041 TGCACACTGT CAGGACCAAT TTACTTGATT TTTGAATACT GTTGCTATGG TGATCTTCTC 2101 AACTATCTAA GAAGTAAAAG AGAAAAATTT CACAGGACTT GGACAGAGAT TTTCAAGGAA 2161 CACAATTTCA GTTTTTACCC CACTTTCCAA TCACATCCAA ATTCCAGCAT GCCTGGTTCA 2221 AGAGAAGTTC AGATACACCC GGACTCGGAT CAAATCTCAG GGCTTCATGG GAATTCATTT 2281 CACTCTGAAG ATGAAATTGA ATATGAAAAC CAAAAAAGGC TGGAAGAAGA GGAGGACTTG 2341 AATGTGCTTA CATTTGAAGA TCTTCTTTGC TTTGCATATC AAGTTGCCAA AGGAATGGAA 2401 TTTCTGGAAT TTAAGTCGTG TGTTCACAGA GACCTGGCCG CCAGGAACGT GCTTGTCACC 2461 CACGGGAAAG TGGTGAAGAT ATGTGACTTT GGATTGGCTC GAGATATCAT GAGTGATTCC 2521 AACTATGTTG TCAGGGGCAA TGCCCGTCTG CCTGTAAAAT GGATGGCCCC CGAAAGCCTG 2581 TTTGAAGGCA TCTACACCAT TAAGAGTGAT GTCTGGTCAT ATGGAATATT ACTGTGGGAA 2641 ATCTTCTCAC TTGGTGTGAA TCCTTACCCT GGCATTCCGG TTGATGCTAA CTTCTACAAA 2701 CTGATTCAAA ATGGATTTAA AATGGATCAG CCATTTTATG CTACAGAAGA AATATACATT 2761 ATAATGCAAT CCTGCTGGGC TTTTGACTCA AGGAAACGGC CATCCTTCCC TAATTTGACT 2821 TCGTTTTTAG GATGTCAGCT GGCAGATGCA GAAGAAGCGA TGTATCAGAA TGTGGATGGC 2881 CGTGTTTCGG AATGTCCTCA CACCTACCAA AACAGGCGAC CTTTCAGCAG AGAGATGGAT 2941 TTGGGGCTAC TCTCTCCGCA GGCTCAGGTC GAAGATTCGT AG
Quality Control: cDNA sequencing results is available upon request.
FLT-3/CD135/FLK-2 related areas, pathways, and other information
