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Human CD135 / FLK2 Gene cDNA Clone (full-length ORF Clone)
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Human CD135 / FLK2 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG10445-M





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

Antibody Production Services

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_004119.2

cDNA Size:   2982

Synonym:  FLK2; STK1; CD135; FLT3

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens fms-related tyrosine kinase 3 DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG


Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for two point mutations: 680 C/T
resulting in the amino acid Thr substitution by Met and 288C/T not causing the amino acid variation.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:

1        ATGCCGGCGT TGGCGCGCGA CGGCGGCCAG CTGCCGCTGC TCGTTGTTTT TTCTGCAATG
61       ATATTTGGGA CTATTACAAA TCAAGATCTG CCTGTGATCA AGTGTGTTTT AATCAATCAT
121      AAGAACAATG ATTCATCAGT GGGGAAGTCA TCATCATATC CCATGGTATC AGAATCCCCG
181      GAAGACCTCG GGTGTGCGTT GAGACCCCAG AGCTCAGGGA CAGTGTACGA AGCTGCCGCT
241      GTGGAAGTGG ATGTATCTGC TTCCATCACA CTGCAAGTGC TGGTCGATGC CCCAGGGAAC
301      ATTTCCTGTC TCTGGGTCTT TAAGCACAGC TCCCTGAATT GCCAGCCACA TTTTGATTTA
361      CAAAACAGAG GAGTTGTTTC CATGGTCATT TTGAAAATGA CAGAAACCCA AGCTGGAGAA
421      TACCTACTTT TTATTCAGAG TGAAGCTACC AATTACACAA TATTGTTTAC AGTGAGTATA
481      AGAAATACCC TGCTTTACAC ATTAAGAAGA CCTTACTTTA GAAAAATGGA AAACCAGGAC
541      GCCCTGGTCT GCATATCTGA GAGCGTTCCA GAGCCGATCG TGGAATGGGT GCTTTGCGAT
601      TCACAGGGGG AAAGCTGTAA AGAAGAAAGT CCAGCTGTTG TTAAAAAGGA GGAAAAAGTG
661      CTTCATGAAT TATTTGGGAT GGACATAAGG TGCTGTGCCA GAAATGAACT GGGCAGGGAA
721      TGCACCAGGC TGTTCACAAT AGATCTAAAT CAAACTCCTC AGACCACATT GCCACAATTA
781      TTTCTTAAAG TAGGGGAACC CTTATGGATA AGGTGCAAAG CTGTTCATGT GAACCATGGA
841      TTCGGGCTCA CCTGGGAATT AGAAAACAAA GCACTCGAGG AGGGCAACTA CTTTGAGATG
901      AGTACCTATT CAACAAACAG AACTATGATA CGGATTCTGT TTGCTTTTGT ATCATCAGTG
961      GCAAGAAACG ACACCGGATA CTACACTTGT TCCTCTTCAA AGCATCCCAG TCAATCAGCT
1021     TTGGTTACCA TCGTAGAAAA GGGATTTATA AATGCTACCA ATTCAAGTGA AGATTATGAA
1081     ATTGACCAAT ATGAAGAGTT TTGTTTTTCT GTCAGGTTTA AAGCCTACCC ACAAATCAGA
1141     TGTACGTGGA CCTTCTCTCG AAAATCATTT CCTTGTGAGC AAAAGGGTCT TGATAACGGA
1201     TACAGCATAT CCAAGTTTTG CAATCATAAG CACCAGCCAG GAGAATATAT ATTCCATGCA
1261     GAAAATGATG ATGCCCAATT TACCAAAATG TTCACGCTGA ATATAAGAAG GAAACCTCAA
1321     GTGCTCGCAG AAGCATCGGC AAGTCAGGCG TCCTGTTTCT CGGATGGATA CCCATTACCA
1381     TCTTGGACCT GGAAGAAGTG TTCAGACAAG TCTCCCAACT GCACAGAAGA GATCACAGAA
1441     GGAGTCTGGA ATAGAAAGGC TAACAGAAAA GTGTTTGGAC AGTGGGTGTC GAGCAGTACT
1501     CTAAACATGA GTGAAGCCAT AAAAGGGTTC CTGGTCAAGT GCTGTGCATA CAATTCCCTT
1561     GGCACATCTT GTGAGACGAT CCTTTTAAAC TCTCCAGGCC CCTTCCCTTT CATCCAAGAC
1621     AACATCTCAT TCTATGCAAC AATTGGTGTT TGTCTCCTCT TCATTGTCGT TTTAACCCTG
1681     CTAATTTGTC ACAAGTACAA AAAGCAATTT AGGTATGAAA GCCAGCTACA GATGGTACAG
1741     GTGACCGGCT CCTCAGATAA TGAGTACTTC TACGTTGATT TCAGAGAATA TGAATATGAT
1801     CTCAAATGGG AGTTTCCAAG AGAAAATTTA GAGTTTGGGA AGGTACTAGG ATCAGGTGCT
1861     TTTGGAAAAG TGATGAACGC AACAGCTTAT GGAATTAGCA AAACAGGAGT CTCAATCCAG
1921     GTTGCCGTCA AAATGCTGAA AGAAAAAGCA GACAGCTCTG AAAGAGAGGC ACTCATGTCA
1981     GAACTCAAGA TGATGACCCA GCTGGGAAGC CACGAGAATA TTGTGAACCT GCTGGGGGCG
2041     TGCACACTGT CAGGACCAAT TTACTTGATT TTTGAATACT GTTGCTATGG TGATCTTCTC
2101     AACTATCTAA GAAGTAAAAG AGAAAAATTT CACAGGACTT GGACAGAGAT TTTCAAGGAA
2161     CACAATTTCA GTTTTTACCC CACTTTCCAA TCACATCCAA ATTCCAGCAT GCCTGGTTCA
2221     AGAGAAGTTC AGATACACCC GGACTCGGAT CAAATCTCAG GGCTTCATGG GAATTCATTT
2281     CACTCTGAAG ATGAAATTGA ATATGAAAAC CAAAAAAGGC TGGAAGAAGA GGAGGACTTG
2341     AATGTGCTTA CATTTGAAGA TCTTCTTTGC TTTGCATATC AAGTTGCCAA AGGAATGGAA
2401     TTTCTGGAAT TTAAGTCGTG TGTTCACAGA GACCTGGCCG CCAGGAACGT GCTTGTCACC
2461     CACGGGAAAG TGGTGAAGAT ATGTGACTTT GGATTGGCTC GAGATATCAT GAGTGATTCC
2521     AACTATGTTG TCAGGGGCAA TGCCCGTCTG CCTGTAAAAT GGATGGCCCC CGAAAGCCTG
2581     TTTGAAGGCA TCTACACCAT TAAGAGTGAT GTCTGGTCAT ATGGAATATT ACTGTGGGAA
2641     ATCTTCTCAC TTGGTGTGAA TCCTTACCCT GGCATTCCGG TTGATGCTAA CTTCTACAAA
2701     CTGATTCAAA ATGGATTTAA AATGGATCAG CCATTTTATG CTACAGAAGA AATATACATT
2761     ATAATGCAAT CCTGCTGGGC TTTTGACTCA AGGAAACGGC CATCCTTCCC TAATTTGACT
2821     TCGTTTTTAG GATGTCAGCT GGCAGATGCA GAAGAAGCGA TGTATCAGAA TGTGGATGGC
2881     CGTGTTTCGG AATGTCCTCA CACCTACCAA AACAGGCGAC CTTTCAGCAG AGAGATGGAT
2941     TTGGGGCTAC TCTCTCCGCA GGCTCAGGTC GAAGATTCGT AG


Quality Control:   cDNA sequencing results is available upon request.

 

FLT-3/CD135/FLK-2 related areas, pathways, and other information

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"