|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
FLT3 was immunoprecipitated using:
Lane A:0.5 mg HepG2 Whole Cell Lysate
Lane B:0.5 mg k562 Whole Cell Lysate2 µL anti-FLT3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-FLT3 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 37 kDa
Observed band size: 37 kDa
Anti-FLT3 rabbit polyclonal antibody at 1:500 dilution
Lane A: Jurkat Whole Cell Lysate
Lane B: HepG2 Whole Cell Lysate
Lane C: K562 Membrane LysateLysates/proteins at 20 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:112 kDa
Observed band size:37 kDa
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD135, also known as FLT-3, FLK-2, is a member of the CD system. CD135 is an important cell surface marker recognized by specific sets of antibodies to identify the types of hematopoietic (blood) progenitors in the bone marrow and it function to differentiate hematopoietic stem cells, which are CD135 negative, from multipotent progenitors, which are CD135 positive. CD135 is a receptor tyrosine kinase typeⅢ for the cytokine Flt3 ligand and activat signaling through second messengers by binding to Flt3. Signaling through CD135 is important for lymphocyte development. The encoding gene CD135 is a proto-oncogene to which mutations happened will lead to cancer such as leukemia.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-FLT3 Antibody (APC)||Mouse||Monoclonal||FCM||10445-MM02-A|
|Anti-FLT3 Antibody (FITC)||Mouse||Monoclonal||FCM||10445-MM02-F|