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FLT-3 / CD135 / FLK-2 Antibody, Rabbit PAb, Antigen Affinity Purified

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Human FLT3 Antibody Product Information
Immunogen:Recombinant Human FLT3 protein (Catalog#10390-H08H)
Clone ID:
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human FLT3 (rh FLT3; Catalog#10390-H08H; NP_116171.3; Met 1-Arg 200). FLT3 specific IgG was purified by human FLT3 affinity chromatography .
Human FLT3 Antibody Usage Guide
Specificity:Human FLT3 / FLK2 / CD135
Application:WB, ELISA, IP

WB: 5-10 μg/mL

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human FLT3. The detection limit for Human FLT3 is approximately 0.00975 ng/well.

IP: 1-4 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human FLT3 Antibody WB Application Image
Human FLT3 Antibody IP Application Image
Other FLT3 Antibody Products
Reactivity: Human  
Application: ELISA  
    200 µg 
    50 µg 
    100 µg 
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    Reactivity: Human  
    Application: ELISA  
      100 µg 
      200 µg 
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      Reactivity: Human  
      Application: FCM  
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      200 µg 
      100 µg 
      50 µg 
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      FLT3/CD135/FLK-2 Background

      The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD135, also known as FLT-3, FLK-2, is a member of the CD system. CD135 is an important cell surface marker recognized by specific sets of antibodies to identify the types of hematopoietic (blood) progenitors in the bone marrow and it function to differentiate hematopoietic stem cells, which are CD135 negative, from multipotent progenitors, which are CD135 positive. CD135 is a receptor tyrosine kinase typeⅢ for the cytokine Flt3 ligand and activat signaling through second messengers by binding to Flt3. Signaling through CD135 is important for lymphocyte development. The encoding gene CD135 is a proto-oncogene to which mutations happened will lead to cancer such as leukemia.

      Human FLT3/CD135/FLK-2 References
    • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
    • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
    • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
    • Bertho, et al. (2000) CD135 (Flk2 / Flt3) Expression by Human Thymocytes Delineates a Possible Role of FLT3-Ligand in T-Cell Precursor Proliferation and Differentiation. Scandinavian Journal of Immunology. 52 (1): 53-61.
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      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.