Anti-FLT3 Antibody

Cat: 10445-RP02

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Anti-FLT3 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-FLT3 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human FLT3
Recombinant Human FLT3 protein (Catalog#10390-H08H)
Produced in rabbits immunized with purified, recombinant Human FLT3 (rh FLT3; Catalog#10390-H08H; NP_116171.3; Met 1-Arg 200). FLT3 specific IgG was purified by human FLT3 affinity chromatography .
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-FLT3 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-FLT3 Antibody (Rabbit Polyclonal antibody) Images
Human FLT3/CD135/FLK-2 Immunoprecipitation(IP) 7986

FLT3 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg k562 Whole Cell Lysate

2 µL anti-FLT3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-FLT3 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 37 kDa

Observed band size: 37 kDa

Human FLT3/CD135/FLK-2 Western blot (WB) 13219

Anti-FLT3 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: HepG2 Whole Cell Lysate

Lane C: K562 Membrane Lysate

Lysates/proteins at 20 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:112 kDa

Observed band size:37 kDa

Anti-FLT3 Antibody Alternative Names
Anti-CD135 Antibody;Anti-FLK-2 Antibody;Anti-FLK2 Antibody;Anti-STK1 Antibody
FLT3 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD135, also known as FLT-3, FLK-2, is a member of the CD system. CD135 is an important cell surface marker recognized by specific sets of antibodies to identify the types of hematopoietic (blood) progenitors in the bone marrow and it function to differentiate hematopoietic stem cells, which are CD135 negative, from multipotent progenitors, which are CD135 positive. CD135 is a receptor tyrosine kinase typeⅢ for the cytokine Flt3 ligand and activat signaling through second messengers by binding to Flt3. Signaling through CD135 is important for lymphocyte development. The encoding gene CD135 is a proto-oncogene to which mutations happened will lead to cancer such as leukemia.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
fms-related tyrosine kinase 3
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Bertho, et al. (2000) CD135 (Flk2 / Flt3) Expression by Human Thymocytes Delineates a Possible Role of FLT3-Ligand in T-Cell Precursor Proliferation and Differentiation. Scandinavian Journal of Immunology. 52 (1): 53-61.
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    Anti-FLT3 Antibody Rabbit Polyclonal ELISA 10445-RP01
    Anti-FLT3 Antibody Mouse Monoclonal ELISA 10445-MM05
    Anti-FLT3 Antibody Mouse Monoclonal FCM 10445-MM02
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