FLRT3 Protein (His Tag)

Leucine-Rich Repeat Transmembrane Protein FLRT3

FLRT3 Protein Product Information

• Synonym: FLRT3
• Protein Construction: A DNA sequence encoding the human FLRT3 ( NP_938205.1 ) extracellular domain ( Met 1 - Pro 528 ) was expressed, fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

FLRT3 Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Lys 28
• Molecular Mass: The recombinant human FLRT3 consists of 511 amino acids and has a predicted molecular mass of 58 kDa. The apparent molecular mass of rh FLRT3 is approximately 60-70 kDa in SDS-PAGE under reducing conditions due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FLRT3 protein

FLRT3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FLRT3 Protein Description

Leucine-rich repeat transmembrane protein FLRT3, also known as Fibronectin-like domain-containing leucine-rich transmembrane protein 3, and FLRT3, is a single-pass type I  membrane protein which belongs to the fibronectin leucine rich transmembrane protein (FLRT) family. FLRT3 contains one fibronectin type-III domain and ten LRR (leucine-rich) repeats and is expressed in kidney, brain, pancreas, skeletal muscle, lung, liver, placenta, and heart. FLRT1, FLRT2 and FLRT3 are members of the FLRT family. They may function in cell adhesion and/or receptor signalling. Their protein structures resemble small leucine-rich proteoglycans found in the extracellular matrix. FLRT3 shares 55% amino acid sequence identity with FLRT1 and 44% identity with FLRT2. Two alternatively spliced transcript variants encoding the same protein have been described. Paraxial protocadherin (PAPC) and FLRT3 form a functional complex with cadherins and PAPC functions as a molecular "governor" to maintain FLRT3 activity at the optimal level for physiological regulation of C-cadherin adhesion, cell sorting, and morphogenesis.

References

1. Otsuki, T. et al., 2007, DNA Res. 12 (2): 117-26.
2. Gerhard, DS. et al., 2004, Genome Res.14 (10B): 2121-7.
3. Robinson, M. et al., 2004, Mol Cell Neurosci. 27 (2): 202-14.
4. Chen, X. et al., 2009, PLoS One. 4 (12): e8411.