FLRT3 Antibody (Antigen Affinity Purified)

FLRT3 Antibody ( Antigen Affinity Purified )

FLRT3 Antibody Product Information

• Immunogen: Recombinant human FLRT3 protein ( Catalog#11166-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human FLRT3 ( rh FLRT3 ; Catalog#11166-H08H ; NP_938205.1 ; Met 1 - Pro 528 ). FLRT3 IgG was purified by human FLRT3 affinity chromatography.

FLRT3 Antibody Usage Guide

• Specificity: Human FLRT3
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human FLRT3 in WB. Using a DAB detection system, the detection limit for human FLRT3 is approximately 2 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human FLRT3. The detection limit for human FLRT3 is 0.00975 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

FLRT3 Antibody Background

Leucine-rich repeat transmembrane protein FLRT3, also known as Fibronectin-like domain-containing leucine-rich transmembrane protein 3, and FLRT3, is a single-pass type I membrane protein which belongs to the fibronectin leucine rich transmembrane protein (FLRT) family. FLRT3 contains one fibronectin type-III domain and ten LRR (leucine-rich) repeats and is expressed in kidney, brain, pancreas, skeletal muscle, lung, liver, placenta, and heart. FLRT1, FLRT2 and FLRT3 are members of the FLRT family. They may function in cell adhesion and/or receptor signalling. Their protein structures resemble small leucine-rich proteoglycans found in the extracellular matrix. FLRT3 shares 55% amino acid sequence identity with FLRT1 and 44% identity with FLRT2. Two alternatively spliced transcript variants encoding the same protein have been described. Paraxial protocadherin (PAPC) and FLRT3 form a functional complex with cadherins and PAPC functions as a molecular "governor" to maintain FLRT3 activity at the optimal level for physiological regulation of C-cadherin adhesion, cell sorting, and morphogenesis.

References

1. Otsuki, T. et al., 2007, DNA Res. 12 (2): 117-26.
2. Gerhard, DS. et al., 2004, Genome Res.14 (10B): 2121-7.
3. Robinson, M. et al., 2004, Mol Cell Neurosci. 27 (2): 202-14.
4. Chen, X. et al., 2009, PLoS One. 4 (12): e8411.