FLRT2 Antibody

FLRT2 Antibody Datasheet

FLRT2 Antibody Product Information

• Immunogen: Recombinant Human FLRT2 protein (Catalog#11296-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 11B5G9
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human FLRT2 (rh FLRT2; Catalog#11296-H08H; O43155; Met 1-Ser 539). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

FLRT2 Antibody Usage Guide

• Specificity: Human FLRT2
• No cross-reactivity in ELISA with

  Human FLRT1

  Human FLRT3

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human FLRT2 in WB. Using a DAB detection system, the detection limit for Human FLRT2 is approximately 4 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human FLRT2. The detection limit for Human FLRT2 is approximately 0.078 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

FLRT2 Antibody Background

Fibronectin Leucine Rich Transmembrane protein 2 (FLRT2) is a Single-pass type I membrane protein. FLRT1, FLRT2 and FLRT3 are belong to Fibronectin Leucine-Rich Transmembrane (FLRT) family, and have been previously reported to be widely expressed in the developing embryo. The FLRT2 proteins contain 10 leucine-rich repeats (LRR), a type III fibronectin (FN) domain, followed by the transmembrane region, and a short cytoplasmic tail. Each member of the FLRT family has a distinct, highly regulated expression pattern, as was seen for the NLRR family. FLRT2 is expressed in a subset of the sclerotome, adjacent to the region that forms the syndetome, suggesting that interaction with FGF signalling may be a general property of FLRT proteins. All FLRTs can interact with FGFR1 and FLRTs can be induced by the activation of FGF signalling by FGF-2. FLRT proteins can physically interact and that FLRT-transfected cultured cells sort out from non-transfected cells, suggesting a change in adhesive properties. FLRT proteins have a dual role, promoting FGF signalling and modulating homotypic cell adhesion.

References

1. Gong SG. et al., 2009, Gene Expr Patterns. 9 (7): 497-502.
2. Maretto S. et al., 2008, Dev Biol. 318 (1): 184-93.
3. Haines BP. et al., 2006, Dev Biol. 297 (1): 14-25.
4. Karaulanov EE. et al., 2006, EMBO Rep. 7 (3): 283-90.