FLRT1 Antibody (Antigen Affinity Purified)

FLRT1 Antibody ( Antigen Affinity Purified ) Datasheet

FLRT1 Antibody Product Information

• Immunogen: Recombinant Human FLRT1 protein (Catalog#11389-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human FLRT1 (rh FLRT1; Catalog#11389-H08H; Q9NZU1-1; Met 1-Pro 524). FLRT1 specific IgG was purified by Human FLRT1 affinity chromatography.

FLRT1 Antibody Usage Guide

• Specificity: Human FLRT1
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human FLRT1 in WB. Using a DAB detection system, the detection limit for Human FLRT1 is approximately 2 ng/lane under non-reducing conditions and 8 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human FLRT1. The detection limit for Human FLRT1 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

FLRT1 Antibody Background

Fibronectin Leucine Rich Transmembrane protein 1 (FLRT1) is a member of the fibronectin leucine rich transmembrane protein (FLRT) family. The three fibronectin leucine-rich repeat transmembrane (FLRT) proteins: FLRT1, FLRT2 and FLRT3, all contain 10 leucine-rich repeats (LRR), a type III fibronectin (FN) domain, followed by the transmembrane region, and a short cytoplasmic tail. FLRTs are glycosylated membrane proteins expressed at the cell surface which localise in a homophilic manner to cell-cell contacts expressing the focal adhesion marker vinculin. Each member of the FLRT family has a distinct, highly regulated expression pattern, as was seen for the NLRR family. FLRT1 is expressed at brain compartmental boundaries. FLRT proteins have dual properties as regulators of cell adhesion and potentiators of fibroblast growth factor (FGF) mediated signalling. FLRT1 is a target for tyrosine phosphorylation mediated by FGFR1 and implicate a non-receptor Src family kinase (SFK). The phosphorylation state of FLRT1, which is itself FGFR1 dependent, may play a critical role in the potentiation of FGFR1 signalling and may also depend on a SFK-dependent phosphorylation mechanism acting via the FGFR. This is consistent with an 'in vivo' role for FLRT1 regulation of FGF signalling via SFKs. Furthermore, the phosphorylation-dependent futile cycle mechanism controlling FGFR1 signalling is concurrently crucial for regulation of FLRT1-mediated neurite outgrowth.

References

1. Maretto S. et al. 2008, Dev Biol. 318 (1): 184-93.
2. Haines BP. et al. 2006, Dev Biol. 297 (1): 14-25.
3. Wheldon LM. et al. 2010, PLoS One. 5 (4): e10264.