FLRG / Fstl3 Antibody

FLRG Antibody Datasheet

FLRG Antibody Product Information

• Immunogen: Recombinant Mouse FLRG protein (Catalog#10591-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 131
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FLRG / Fstl3 (rM FLRG / Fstl3; Catalog#10591-M08H; NP_113557.1; Met 1-Val 256).

FLRG Antibody Usage Guide

• Specificity: Mouse FLRG
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse FLRG. The detection limit for Mouse FLRG is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

FLRG Antibody Background

FLRG (follistatin-related gene) protein is a follistatin-like protein which contains two FS domains and acts as a binding protein for activin and BMP-2. FLRG is a secreted glycoprotein that is highly homologous with follistatin. These proteins are involved in the regulation of various biological effects mediated by their binding to TGF-β superfamily members, activin A and bone morphogenetic proteins.The mouse FLRG gene consists of five exons, and each encodes discrete functional regions.Although mouse and human FLRGs show some divergence compared with follistatins, structurally they are conserved, also, domain organization and putative Asn-linked glycosylation sites are completely conserved. Both mouse and human FLRG have an NH2-terminal domain, two FS domains that are thought to have growth factor-binding motifs, and a COOH-terminal acidic domain. Recombinant mouse FLRG protein binds to activin A and BMP-2, and blocked the activities of acitvin A, activin B, activin AB, and BMP-2. Mouse FLRG mRNA is abundantly expressed in heart, lung, kidney, and testis. FLRG is also expressed in the mouse ovary, suggesting that FLRG plays physiological roles in the regulation of ovarian function as follistatin does.

References

1. Laurent Bartholin, et al., 2005, Biology of the Cell, 97: 577-588.
2. Masashi Nakatani, et al., 2002, Molecular and Cellular Endocrinology, Volume 189, Issues 1-2: 117-123.
3. Koji Y. Arai, et al., 2003, Biology of reproduction, 68: 199-206.
4. Kunihiro Tsuchida, et al., 2000, The Journal of Biological Chemistry, Vol. 275, No. 52: 40788-40796.