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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human FKBP7 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG13250-G-F|
|Human FKBP7 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG13250-G-H|
|Human FKBP7 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG13250-G-M|
|Human FKBP7 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG13250-G-N|
|Human FKBP7 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG13250-G-Y|
PPIase is a member of the immunophilin protein family. It also belongs to the cyclophilin-type PPIase family, PPIL3 subfamily. PPIase contains 1 PPIase cyclophilin-type domain. Members of the immunophilin protein family play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. It has a very high substrate specificity for the four-residue peptide Ala-Ala-Pro-Phe only when the proline peptide bond is in the trans state. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium.