Anti-FKBP12 Antibody

Cat: 10268-RP02
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Anti-FKBP12 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-FKBP12 Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human FKBP12
Immunogen
Recombinant Human FKBP1A protein (Catalog#10268-H08E)
Preparation
Produced in rabbits immunized with purified, recombinant Human FKBP1A (rh FKBP1A; Catalog#10268-H08E; NP_463460; Met 1-Glu 108). FKBP1A specific IgG was purified by human FKBP1A affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-FKBP12 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-FKBP12 Antibody (Rabbit Polyclonal antibody) Images
Human FKBP12/FKBP1A Western blot (WB) 13158

Anti-FKBP1A rabbit polyclonal antibody at 1:500 dilution

Lane A: SH-SY5Y Whole Cell Lysate

Lane B: HL-60 Whole Cell Lysate

Lane C: U937 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:12 kDa

Observed band size:12 kDa

(We are unsure as to the identity of these extra bands.)

Anti-FKBP12 Antibody Alternative Names
Anti-FKBP-12 Antibody;Anti-FKBP-1A Antibody;Anti-FKBP1 Antibody;Anti-FKBP12 Antibody;Anti-PKC12 Antibody;Anti-PKCI2 Antibody;Anti-PPIASE Antibody
FKBP12 Background Information

FK506 binding protein 12 (FKBP12), also known as FKBP1, along with cyclophilin, are two major members of the immunophilin protein family who serve as receptors for the immunosuppressant drugs cyclosporin A and FK506. As a conserved molecules in many eukaryotes, FKBP12 has been characterized as a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues, and is involved in several biochemical processes including protein folding, receptor signaling, protein trafficking and transcription. FKBP12 has attracted immense attention and its role in mediating the immunosuppressive functions. FKBP12 serves a dual role as a peptidyl-prolyl cis-trans isomerase and as a modulator of several cell signaling pathways. In one such a role, FKBP12 interacts with and regulates the functional state of the ryanodine Ca2+ channel receptor by altering protein conformation and coordinating multi-protein complex formation. Another physiological role of FKBP12 is an interactor and a regulator of the type I serine/threonine kinase receptors of TGF-beta superfamily. Current data, derived from detailed biochemical studies as well as from functional studies in various systems, suggest that FKBP12 functions as a "guardian" for the type I receptors to prevent them from leaky signaling under sub-optimal ligand concentrations, thereby providing a molecular "gradient reader" for TGF-beta family morphogens. This aspect of FKBP12 function may be critical for cellular responsiveness to morphogenetic gradients of the TGF-beta family members during early development, serving to assure the translation of different ligand concentrations into different signaling readouts. In addition, FKBP12 may be involved in neuronal or astrocytic cytoskeletal organization and in the abnormal metabolism of tau protein in Alzheimer's disease (AD) damaged neurons.

Full Name
FK506 binding protein 1A
References
  • Wang T, et al. (2004) The immunophilin FKBP12: a molecular guardian of the TGF-beta family type I receptors. Front Biosci. 9: 619-31.
  • Sugata H, et al. (2009) A peptidyl-prolyl isomerase, FKBP12, accumulates in Alzheimer neurofibrillary tangles. Neurosci Lett. 459(2): 96-9.
  • Brath U, et al. (2009) Differential responses of the backbone and side-chain conformational dynamics in FKBP12 upon binding the transition-state analog FK506: implications for transition-state stabilization and target protein recognition. J Mol Biol. 387(1): 233-44.
  • Scaramello CB, et al.. (2009) FKBP12 depletion leads to loss of sarcoplasmic reticulum Ca(2+) stores in rat vas deferens. J Pharmacol Sci. 109(2): 185-92.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-FKBP12 Antibody Mouse Monoclonal WB,ELISA 10268-MM02
    Anti-FKBP12 Antibody Rabbit Polyclonal ELISA 10268-RP01
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