Mouse FGFRL1 / FGFR5 Protein (His Tag)

Mouse Fibroblast Growth Factor Receptor-like 1 Protein

FGFRL1 / FGFR5 Protein Product Information

• Synonym: Fgfrl1, FGFR5, FGFR5beta, FGFR5gamma
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse FGFRL1 ( NP_473412.1 ) ( Met 1 - Pro 374 ) precursor was expressed, with a C-terminal polyhistidine tag
• Source: Mouse
• Expression Host: Human Cells

FGFRL1 / FGFR5 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Bio-activity: Measured by its binding ability in a functional ELISA
  1. Immobilized mouse at 10 μg/ml ( 100 μl/well ) can bind mouse FGFR5. The EC₅₀ of mouse FGFR5 is 0.34 μg/mL
  2. Immobilized human FGF1 at 10μg/ml ( 100 μl/well ) can bind mouse FGFR5 with a linear range of 0.08 - 2 μg/ml
  3. Immobilized human bFGF at 5 μg/ml ( 100 μl/well ) can bind mouse FGFR5. The EC₅₀ of mouse FGFR5 is 0.22 μg/mL
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 21
• Molecular Mass: The secreted recombinant mouse FGFRL1 consists of 365 amino acids and has a calculated molecular mass of 40.4 kDa. The apparent molecular mass of rmFGFRL1 is approximately 55-60 KDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FGFRL1 protein

FGFRL1 / FGFR5 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FGFRL1 / FGFR5 Protein Description

Mouse Fibroblast growth factor receptor-like 1, also known as FGF receptor-like protein 1, Fibroblast growth factor receptor 5, FGFR5, and FGFRL1, is a single-pass type I  membrane protein. FGFR5 is expressed preferentially in cartilaginous tissues and pancreas. It is highly expressed in the liver, kidney, heart, brain and skeletal muscle and weakly expressed in the lung, small intestine and spleen. FGFR5 contains three Ig-like C2-type (immunoglobulin-like) domains. A unique feature of FGFR5 is that it does not contain an intracellular tyrosine kinase domain. While mammals, including man and mouse, possess a single copy of the FGFRL1 gene, fish have at least two copies, FGFRL1a and FGFRL1b. Fibroblast Growth Factors (FGF) and their receptors are well known for having major implications in cell signalling controlling embryonic development. FGFR5 has a negative effect on cell proliferation. It is first detected at embryonic day 7 and became clearly visible at day 11 and increased until day 17. FGFR5 may act as a decoy receptor for FGF ligands.

References

1. Murao, K. et al.,1997, J. Biol. Chem. 272 (28): 17551-7. 
2. Sleeman, M. et al., 2001, Gene. 271 (2):171-82.
3. Trueb, B. et al., 2003, J Biol Chem. 278 (36): 33857-65.
4. Trueb, B. et al., 2005, Biochim Biophys Acta. 1727 (1): 65-74.
5. Bertrand, S. et al., 2009, BMC Evol Biol . 9 : 226.