FGFRL1 / FGFR5 Antibody

FGFRL1 / FGFR5 Antibody Datasheet

FGFRL1 / FGFR5 Antibody Product Information

• Immunogen: Recombinant Mouse FGFRL1 / FGFR5 Protein (Catalog#50182-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 004
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Mouse FGFRL1 / FGFR5 (rM FGFRL1 / FGFR5; Catalog#50182-M08H; NP_473412.1; Met 1 - Pro 374).

FGFRL1 / FGFR5 Antibody Usage Guide

• Specificity: Mouse FGFRL1 / FGFR5
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse FGFRL1 in WB. Using a DAB detection system, the detection limit for Mouse FGFRL1 is approximately 0.25 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse FGFRL1. The detection limit for Mouse FGFRL1 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

FGFRL1 / FGFR5 Antibody Background

Mouse Fibroblast growth factor receptor-like 1, also known as FGF receptor-like protein 1, Fibroblast growth factor receptor 5, FGFR5, and FGFRL1, is a single-pass type I  membrane protein. FGFR5 is expressed preferentially in cartilaginous tissues and pancreas. It is highly expressed in the liver, kidney, heart, brain and skeletal muscle and weakly expressed in the lung, small intestine and spleen. FGFR5 contains three Ig-like C2-type (immunoglobulin-like) domains. A unique feature of FGFR5 is that it does not contain an intracellular tyrosine kinase domain. While mammals, including man and mouse, possess a single copy of the FGFRL1 gene, fish have at least two copies, FGFRL1a and FGFRL1b. Fibroblast Growth Factors (FGF) and their receptors are well known for having major implications in cell signalling controlling embryonic development. FGFR5 has a negative effect on cell proliferation. It is first detected at embryonic day 7 and became clearly visible at day 11 and increased until day 17. FGFR5 may act as a decoy receptor for FGF ligands.  

References

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5. Bertrand, S. et al., 2009, BMC Evol Biol . 9 : 226.