Rat FGFR4 / FGF Receptor 4 Protein

Fibroblast growth factor receptor 4 Protein Datasheet

FGFR4 / FGF Receptor 4 Protein Product Information

• Synonym: FGFR-4
• Protein Construction: A DNA sequence encoding the rat FGFR4 (Q498D6) extracellular domain (Met 1-Asp 367) was fused with the Fc region of human IgG1 at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

FGFR4 / FGF Receptor 4 Protein QC Testing

• Purity: >90 % as determined by SDS-PAGE.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized human FGF18 (Cat: 13206-H08H) at 10 μg/mL (100 μl/well) can bind Rat FGFR4 (Cat:80093-R02H), The EC₅₀ of Rat FGFR4 (Cat:80093-R02H) is 1.17 μg/mL.
  3. Immobilized mouse FGF18 (Cat:50177-M08H) at 10 μg/mL (100 μl/well) can bind Rat FGFR4 (Cat:80093-R02H), The EC₅₀ of Rat FGFR4 (Cat:80093-R02H) is 0.44 μg/mL.
  4. Immobilized human bFGF at 10 μg/mL (100 μl/well) can bind Rat FGFR4 (Cat:80093-R02H), The EC₅₀ of Rat FGFR4 (Cat:80093-R02H) is 0.163 μg/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Phe 17
• Molecular Mass: The recombinant rat FGFR4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 592 amino acids and predicts a molecular mass of 66 kDa. The apparent molecular mass of the rat FGFR4/Fc monomer is approximately 110 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FGFR4 / FGF Receptor 4 protein

FGFR4 / FGF Receptor 4 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FGFR4 / FGF Receptor 4 Protein Description

FGFR4, also designated as CD334, is a member of the fibroblast growth factor receptor subfamily which belongs to the tyrosine protein kinase superfamily. Upon ligand binding, FGFRs undergo dimerization and autophosphorylation, intiating a cascade of downstream signal pathways, ultimately influencing a variety of physiological and pathological cellular processes including mitogenesis and differentiation. FGFR members is highly conserved in amino acid sequence and differ in their ligand affinities and tissue distribution. FGFR4, a type I transmembrane glycoprotein, contains 3 immunoglobulin-like domains in the extracellular domain and a cytoplasmic tyrosine-kinase domain. FGFR4 expression is detected in gastrointestinal epithelial cells, pancreas, as well as gastric and pancreatic cancer cell lines. FGFR4 is reported to be a high affinity receptor for both acidic and basic fibroblast growth factor, and also binds to FGF19. Like FGFR1-3, several isoforms of FGFR4 are generated by alternative splicing of the mRNAs. Although its specific function is unknown, growing studies have indicated that FGFR4 plays a role in tumorigenesis and malignace of cetain carcinomas such as breast and ovarian tumors.

References

1. Partanen J. et al., 1991, EMBO J. 10: 1347-54.
2. Ron D. et al., 1993, J Biol Chem. 268: 5388-94.
3. Marics I. et al., 2002, Development. 129: 4559-69.
4. Bnage J. et al., 2002, Cancer Res. 62: 840-7.