FGFR2 / CD332 Protein (His Tag)

Fibroblast Growth Factor Receptor 2 ( FGFR2 / CD332 ) Protein

FGFR2 / CD332 Protein Product Information

• Synonym: BEK, BFR-1, CD332, CEK3, CFD1, ECT1, FLJ98662, JWS, K-SAM, KGFR, TK14, TK25
• Protein Construction: A DNA sequence encoding the human FGFR2 ( NP_000132.3 ) extracellular domain ( Met 1 - Glu 377 ) was expressed, fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

FGFR2 / CD332 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to inhibit FGF acidic dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts.
  The ED₅₀ for this effect is typically 200 - 400 ng/mL
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Arg 22
• Molecular Mass: The recombinant human FGFR2 consists of 367 amino acids after removal of the signal peptide and has a calculated molecular mass of 41 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhFGFR2 is approximately 65-75 kDa due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FGFR2 protein

FGFR2 / CD332 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FGFR2 / CD332 Protein Description

Fibroblast growth factor receptor 2 (FGFR2) is a member of the fibroblast growth factor receptor (FGFR) subfamily belonging to the Tyr protein kinase family, and amino acid sequence of which is highly conserved between members throughout evolution. FGFR family members differ from one another in their ligand affinities , tissue distribution and have been implicated in a multitude of activities. FGFR2 is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. This protein consists of an extracellular region composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. Mutations in the FGFR2 gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis.

References

1. Santos-Ocampo, S. et al., 1996, J. Biol. Chem. 271 (3): 1726-31.
2. Stauber, D. J. et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97 (1): 49-54.
3. Pellegrini, L. et al., 2000, Nature. 407 (6807): 1029-34.
4. Yu, S. et al., 2002, Am J Physiol Endocrinol Metab. 283 (3): E490-5.
5. Hunter, D.J. et al., 2007, Nature Genetics. 39: 870-4.