FGFR2 / CD332 Antibody (Antigen Affinity Purified)

Fibroblast Growth Factor Receptor 2 ( FGFR2 / CD332 ) Antibody

FGFR2 / CD332 / BEK Antibody Product Information

• Immunogen: Recombinant human FGFR2 protein ( Catalog#10824-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human FGFR2 extracellular domain ( rh FGFR2 ; Catalog#10824-H08H ; NP_000132.3 ; Met 1 - Glu 377 ). FGFR2 specific IgG was purified by human FGFR2 affinity chromatography.

FGFR2 / CD332 / BEK Antibody Usage Guide

• Specificity: Human FGFR2 / CD332 / BEK
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human FGFR2 in WB. Using a DAB detection system, the detection limit for human FGFR2 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.5 -1.0 μg/mL with the appropriate secondary reagents to detect human FGFR2. The detection limit for human FGFR2 is 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

FGFR2 / CD332 / BEK Antibody Background

Fibroblast growth factor receptor 2 (FGFR2) is a member of the fibroblast growth factor receptor (FGFR) subfamily belonging to the Tyr protein kinase family, and amino acid sequence of which is highly conserved between members throughout evolution. FGFR family members differ from one another in their ligand affinities , tissue distribution and have been implicated in a multitude of activities. FGFR2 is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. This protein consists of an extracellular region composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. Mutations in the FGFR2 gene are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis.

References

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