FGFR1 / CD331 Protein (His Tag)

Fibroblast Growth Factor Receptor 1 ( FGFR1 / CD331 ) Protein

FGFR1 / CD331 Protein Product Information

• FGFR1 Synonym: FGFR1, BFGFR, CD331, CEK, FGFBR, FLG, FLJ99988, FLT2, HBGFR, KAL2, N-SAM
• Protein Construction: A DNA sequence encoding the human FGFR1 ( NP_075594.1 ) extracellular domain ( Met 1 - Glu 285 ) was expressed with C-terminal polyhistidine tag
• Source: Human
• Expression Host: Human Cells

FGFR1 / CD331 Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to inhibit aFGF / FGF-acidic dependent proliferation of BALB / 3T3 mouse fibroblasts
  The ED₅₀ for this effect is typically 8-40 ng/ml
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Arg 22
• Molecular Mass: The secreted recombinant human FGFR1 consists of 275 amino acids and has a predicted molecular mass of 31 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh FGFR1 monomer is approximately 50-55 kDa due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FGFR1 protein

FGFR1 / CD331 Protein Usage Guide

• Storage: Store it under sterile conditions at -70. It is recommended that the protein be aliquoted for optimal storage and be used as soon as possible. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FGFR1 / CD331 Protein Description

Fibroblast growth factor receptor 1 (FGFR1) is a member of the fibroblast growth factor receptor (FGFR) family, whose amino acid sequence is highly conserved between members throughout evolution. FGFR family members differ from one another in their ligand affinities , tissue distribution and have been implicated in a multitude of activities. FGFR1, also known as fms-related tyrosine kinase-2 (FLT2) and CD331, is a receptor tyrosine kinase whose ligands are specific members of the fibroblast growth factor family. This protein structurally consists of an extracellular region containing three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR1 is the receptor for FGF basic and also interacts with GRB14, SHB, FRS2. Defects in FGFR1 are the cause of several deseases, such as Kallmann syndrome type 2 (KAL2), osteoglophonic dysplasia (OGD), as well as Pfeiffer syndrome (PF).

References

1. Santos-Ocampo, S. et al., 1996, J. Biol. Chem. 271 (3): 1726-1731.
2. Stauber, D. J. et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97 (1): 49-54.
3. Pellegrini, L. et al., 2000, Nature. 407 (6807): 1029-1034.
4. Yu, S. et al., 2002, Am J Physiol Endocrinol Metab. 283 (3): E490-495.
5. Hunter, D.J. et al., 2007, Nature Genetics. 39: 870-874.