FGF19 Protein (Native)

Fibroblast growth factor 19 Protein Datasheet

FGF19 Protein Product Information

• Synonym: FGF19
• Protein Construction: A DNA sequence encoding the mature form of human FGF19 (O95750) (Phe27-Lys216) was expressed, with a N-terminal Met.
• Source: Human
• Expression Host: E.coli

FGF19 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human FGF19 consists of 191 amino acids and predicts a molecular mass of 21.4 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 15% gly 50mM Tris 2mM EDTA.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FGF19 protein

FGF19 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FGF19 Protein Description

FGF19, also known as FGF-19, belongs to the fibroblast growth factor (FGF) family. Members of this family possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF19 is involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. It also stimulates glucose uptake in adipocytes. It is expressed in fetal brain, cartilage, retina, and adult gall bladder. FGF19 interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by KL, KLB and heparan sulfate glycosaminoglycans that function as coreceptors. It interacts with KL and KLB directly. However, it interacts with FGFR4 in the presence of heparin, KL or KLB.

References

1. Tamimi Y. et al., 2006, Hum Mol Genet. 15 (21): 3229-40.
2. Goetz R. et al., 2007, Mol Cell Biol. 27 (9): 3417-28.
3. Harmer NJ. et al., 2004, Biochemistry. 43 (3): 629-40.