FGF18 / FGF-18 Protein (His Tag)

Fibroblast growth factor 18 Protein Datasheet

FGF18 / FGF-18 Protein Product Information

• Synonym: UNQ420, PRO856, FGF-18, ZFGF5
• Protein Construction: A DNA sequence encoding the human FGF18 (O76093) (Met 1- Ala 207) was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

FGF18 / FGF-18 Protein QC Testing

• Purity: >92 % as determined by SDS-PAGE.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. 2. Immobilized human FGF18 (Cat: 13206-H08H) at 10 μg/mL (100 μl/well) can bind Rat FGFR4 (Cat:80093-R02H), The EC₅₀ of Rat FGFR4 (Cat:80093-R02H) is 1.17 μg/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 28
• Molecular Mass: The secreted recombinant human FGF18 comprises 191 amino acids and has a predicted molecular mass of 22.3 kDa. The apparent molecular mass of rh FGF18 is approximately 32 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FGF18 / FGF-18 protein

FGF18 / FGF-18 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FGF18 / FGF-18 Protein Description

Fibroblast growth factor 18, also known as FGF-18, and FGF18, is a secreted protein which belongs to the fibroblast growth factors (FGFs) family. FGFs and their high-affinity receptors contribute to the autocrine growth stimulation in several malignancies. FGF18 is abundantly expressed in mouse embryonic lungs. It plays roles in lung alveolar development during late embryonic lung development stages. The cell proliferation during the terminal saccular stage stimulated by FGF18 might play roles in the remodeling of the distal lung. FGF18 expression is up-regulated in colorectal tumours and is progressively enhanced during colon carcinogenesis reaching very high levels in carcinoma. FGF18 affects both tumour cells and tumour microenvironment in a pro-tumorigenic and pro-metastatic way. FGF18 is almost generally over-expressed in colon cancer and exerts pro-tumorigenic effects both in the epithelial and the stromal compartments by stimulating growth and survival of tumour cells, migration of fibroblasts and neovascularization. FGF18 has been shown to regulate chondrocyte proliferation and differentiation by signaling through FGF receptor 3 (FGFR3) and to regulate osteogenesis by signaling through other FGFRs. FGF18 regulates skeletal vascularization and subsequent recruitment of osteoblasts / osteoclasts through regulation of early stages of chondrogenesis and VEGF expression. FGF18 coordinates neovascularization of the growth plate with chondrocyte and osteoblast growth and differentiation.

References

1. Ohbayashi N. et al., 2002, Genes Dev. 16 (7): 870-9.
2. Usui H. et al., 2004, Biochem Biophys Res Commun. 322 (3): 887-92.
3. Liu Z. et al., 2007, Dev Biol. 302 (1): 80-91.
4. Reinhold MI. et al., 2007, J Biol Chem. 282 (6): 3653-63.
5. Sonvilla G. et al., 2008, Carcinogenesis. 29 (1):15-24.