Mouse FGF15 Gene cDNA Clone / ORF Clone

FGF15 cDNA Clone Datasheet

FGF15 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_008003.2
• cDNA Size: 657
• Gene Synonym: FGF19; Fgf15
• Source: Mouse

FGF15 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus fibroblast growth factor 15 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 87 T/C not causing the amino acid variation.

FGF15 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FGF15 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FGF15 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGAGAA AGTGGAACGG GCGTGCGGTG GCCCGAGCCC TGGTCCTGGC CACTCTGTGG
61       CTGGCTGTGT CTGGGCGTCC CCTGGCCCAG CAATCCCAGT CTGTGTCAGA TGAAGATCCA
121      CTCTTTCTCT ACGGCTGGGG CAAGATTACC CGCCTGCAGT ACCTGTACTC CGCTGGTCCC
181      TATGTCTCCA ACTGCTTCCT CCGAATCCGG AGCGACGGCT CTGTGGACTG CGAGGAGGAC
241      CAAAACGAAC GAAATTTGTT GGAATTCCGC GCGGTCGCTC TGAAGACGAT TGCCATCAAG
301      GACGTCAGCA GCGTGCGGTA CCTCTGCATG AGCGCGGACG GCAAGATATA CGGGCTGATT
361      CGCTACTCGG AGGAAGACTG TACCTTCAGG GAGGAAATGG ACTGTTTAGG CTACAACCAG
421      TACAGATCCA TGAAGCACCA TCTCCATATC ATCTTCATCC AGGCCAAGCC CAGAGAACAG
481      CTCCAGGACC AGAAACCCTC AAACTTTATC CCCGTGTTTC ACCGCTCCTT CTTTGAAACC
541      GGGGACCAGC TGAGGTCTAA AATGTTCTCC CTGCCCCTGG AGAGTGACAG CATGGATCCG
601      TTCAGGATGG TGGAGGATGT AGACCACCTA GTGAAGAGTC CCAGCTTCCA GAAATGA

 

Quality Control:cDNA sequencing results is available upon request.