Cynomolgus monkey FGF14 Gene cDNA Clone / ORF Clone

FGF14 cDNA Clone Datasheet

FGF14 cDNA Clone Product Information

• cDNA Size: 744
• Gene Synonym: FGF14
• Source: Cynomolgus monkey

FGF14 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) fibroblast growth factor 14 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 618 T/C not causing the amino acid variation.
  Please check the sequence information before order.

FGF14 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FGF14 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FGF14 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCGCGG CCATCGCCAG CGGCTTGATC CGCCAGAAGC GGCAGGCGCG GGAGCAGCAC
61       TGGGACCGGC CGTCTGCCAG CAGGAGGCGG AGCAGCCCCA GCAAGAACCG CGGGCTCTGC
121      AACGGCAACC TGGTGGATAT CTTCTCCAAA GTGCGCATCT TCGGTCTCAA GAAGCGCAGG
181      TTGCGGCGCC AAGATCCCCA GCTCAAGGGT ATAGTGACCA GGTTATATTG CAGGCAAGGC
241      TACTACTTGC AAATGCACCC CGATGGAGCT CTCGATGGAA CCAAGGATGA CAGCACTAAT
301      TCTACGCTCT TCAACCTCAT TCCAGTGGGA CTGCGTGTTG TTGCCATCCA GGGAGTGAAA
361      ACAGGGTTGT ATATAGCCAT GAATGGAGAA GGTTACCTCT ACCCATCAGA ACTTTTTACC
421      CCTGAATGCA AGTTTAAAGA ATCTGTTTTT GAAAATTATT ATGTAATCTA CTCATCCATG
481      CTGTACAGAC AACAGGAATC TGGTAGAGCC TGGTTTTTGG GATTAAATAA GGAAGGGCAA
541      GCTATGAAAG GGAACAGAGT AAAGAAAACC AAACCAGCAG CTCATTTTCT ACCCAAGCCA
601      TTGGAAGTTG CCATGTACCG AGAACCATCT TTGCATGATG TTGGGGAAAC GGTCCCGAAG
661      CCTGGGGTGA CGCCAAGTAA AAGCACAAGT GCGTCTGCAA TAATGAATGG AGGCAAACCA
721      GTCAACAAGA GTAAGACAAC ATAG

 

Quality Control:cDNA sequencing results is available upon request.