Cynomolgus monkey FGF12 Gene cDNA Clone / ORF Clone

FGF12 cDNA Clone Datasheet

FGF12 cDNA Clone Product Information

• cDNA Size: 732
• Gene Synonym: FGF12
• Source: Cynomolgus monkey

FGF12 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) fibroblast growth factor 12 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 580 A/C resulting in the amino acid Thr substitution by Pro and 78 T/C，258 A/G，579 A/G not causing the amino acid variation.
  Please check the sequence information before order.

FGF12 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FGF12 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FGF12 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGCGG CGATAGCCAG CTCCTTGATC CGACAGAAGC GGCAGGCGAG GGAGTCCAAC
61       AGCGACCGAG TGTCGGCCTC CAAGCGCCGC TCCAGCCCCA GCAAAGACGG GCGCTCCCTG
121      TGCGAGAGGC ACGTCCTCGG GGTGTTCAGC AAAGTGCGCT TCTGCAGCGG CCGCAAGAGG
181      CCGGTGAGGC GGAGACCAGA ACCCCAGCTG AAAGGGATTG TGACAAGGTT ATTCAGCCAG
241      CAGGGATATT TCCTGCAGAT GCACCCAGAT GGCACCATTG ATGGGACCAA GGACGAAAAC
301      AGCGACTACA CTCTCTTCAA TCTAATTCCC GTGGGCCTGC GTGTAGTGGC CATCCAAGGA
361      GTGAAGGCTA GCCTCTATGT GGCCATGAAT GGTGAAGGCT ATCTCTACAG TTCTGATGTT
421      TTCACTCCAG AATGCAAATT TAAGGAATCT GTGTTTGAAA ACTACTATGT GATCTATTCT
481      TCCACACTGT ACCGCCAGCA AGAATCAGGC CGAGCTTGGT TTCTGGGACT CAATAAAGAA
541      GGTCAAATTA TGAAGGGGAA CAGAGTGAAG AAAACCAAGC CCTCATCACA TTTTGTACCG
601      AAACCTATTG AAGTGTGTAT GTACAGAGAA CCATCACTAC ATGAAATTGG AGAAAAACAA
661      GGGCGTTCAA GGAAAAGTTC TGGAACACCA ACCATGAATG GAGGCAAAGT TGTGAATCAA
721      GATTCAACAT AG

 

Quality Control:cDNA sequencing results is available upon request.