Human FFAR1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13526-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

FFAR1 cDNA Clone Datasheet

FFAR1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_005303.2
cDNA Size:	903
Gene Synonym:	FFA1R; GPR40; GPCR40；FFAR1
Source:	Human

FFAR1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens free fatty acid receptor 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 632 G/A resulting in the amino acid Arg substitution by His.

FFAR1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FFAR1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FFAR1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACCTGC CCCCGCAGCT CTCCTTCGGC CTCTATGTGG CCGCCTTTGC GCTGGGCTTC
61       CCGCTCAACG TCCTGGCCAT CCGAGGCGCG ACGGCCCACG CCCGGCTCCG TCTCACCCCT
121      AGCCTGGTCT ACGCCCTGAA CCTGGGCTGC TCCGACCTGC TGCTGACAGT CTCTCTGCCC
181      CTGAAGGCGG TGGAGGCGCT AGCCTCCGGG GCCTGGCCTC TGCCGGCCTC GCTGTGCCCC
241      GTCTTCGCGG TGGCCCACTT CTTCCCACTC TATGCCGGCG GGGGCTTCCT GGCCGCCCTG
301      AGTGCAGGCC GCTACCTGGG AGCAGCCTTC CCCTTGGGCT ACCAAGCCTT CCGGAGGCCG
361      TGCTATTCCT GGGGGGTGTG CGCGGCCATC TGGGCCCTCG TCCTGTGTCA CCTGGGTCTG
421      GTCTTTGGGT TGGAGGCTCC AGGAGGCTGG CTGGACCACA GCAACACCTC CCTGGGCATC
481      AACACACCGG TCAACGGCTC TCCGGTCTGC CTGGAGGCCT GGGACCCGGC CTCTGCCGGC
541      CCGGCCCGCT TCAGCCTCTC TCTCCTGCTC TTTTTTCTGC CCTTGGCCAT CACAGCCTTC
601      TGCTACGTGG GCTGCCTCCG GGCACTGGCC CACTCCGGCC TGACGCACAG GCGGAAGCTG
661      CGGGCCGCCT GGGTGGCCGG CGGGGCCCTC CTCACGCTGC TGCTCTGCGT AGGACCCTAC
721      AACGCCTCCA ACGTGGCCAG CTTCCTGTAC CCCAATCTAG GAGGCTCCTG GCGGAAGCTG
781      GGGCTCATCA CGGGTGCCTG GAGTGTGGTG CTTAATCCGC TGGTGACCGG TTACTTGGGA
841      AGGGGTCCTG GCCTGAAGAC AGTGTGTGCG GCAAGAACGC AAGGGGGCAA GTCCCAGAAG
901      TAA

Quality Control:cDNA sequencing results is available upon request.