Human FES Kinase/Feline sarcoma oncogene Gene cDNA Clone / ORF Clone

FES cDNA Clone Datasheet

FES cDNA Clone Product Information

• Gene Bank Ref.ID: BC035357
• cDNA Size: 2469
• Gene Synonym: FPS;FES
• Source: Human

FES cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens feline sarcoma oncogene DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

FES cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FES cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FES cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCTTCT CTTCTGAGCT GTGCAGCCCC CAGGGCCACG GGGTCCTGCA GCAAATGCAG
61       GAGGCCGAGC TTCGTCTACT GGAGGGCATG AGAAAGTGGA TGGCCCAGCG GGTCAAGAGT
121      GACAGGGAGT ATGCAGGACT GCTTCACCAC ATGTCCCTGC AGGACAGTGG GGGCCAGAGC
181      CGGGCCATCA GCCCTGACAG CCCCATCAGT CAGTCCTGGG CTGAGATCAC CAGCCAAACT
241      GAGGGCCTGA GCCGCTTGCT GCGGCAGCAC GCAGAGGATC TGAACTCAGG GCCCCTGAGC
301      AAGCTGAGCC TGCTCATCCG GGAACGGCAG CAGCTTCGCA AGACCTACAG CGAGCAGTGG
361      CAGCAGCTGC AGCAGGAGCT CACCAAGACC CACAGCCAGG ACATTGAGAA GCTGAAGAGC
421      CAGTACCGAG CTCTGGCACG GGACAGTGCC CAAGCCAAGC GCAAGTACCA GGAGGCCAGC
481      AAAGACAAGG ACCGTGACAA GGCCAAGGAC AAGTATGTGC GCAGCCTGTG GAAGCTCTTT
541      GCTCACCACA ACCGCTATGT GCTGGGCGTG CGGGCTGCGC AGCTACACCA CCAGCACCAC
601      CACCAGCTCC TGCTGCCCGG CCTGCTGCGG TCACTGCAGG ACCTGCACGA GGAGATGGCT
661      TGCATCCTGA AGGAGATCCT GCAGGAATAC CTGGAGATTA GCAGCCTGGT GCAGGATGAG
721      GTGGTGGCCA TTCACCGGGA GATGGCTGCA GCTGCTGCCC GCATCCAGCC TGAGGCTGAG
781      TACCAAGGCT TCCTGCGACA GTATGGGTCC GCACCTGACG TCCCACCCTG TGTCACGTTC
841      GATGAGTCAC TGCTTGAGGA GGGTGAACCG CTGGAGCCTG GGGAGCTCCA GCTGAACGAG
901      CTGACTGTGG AGAGCGTGCA GCACACGCTG ACCTCAGTGA CAGATGAGCT GGCTGTGGCC
961      ACCGAGATGG TGTTCAGGCG GCAGGAGATG GTTACGCAGC TGCAACAGGA GCTCCGGAAT
1021     GAAGAGGAGA ACACCCACCC CCGGGAGCGG GTGCAGCTGC TGGGCAAGAG GCAAGTGCTG
1081     CAAGAAGCAC TGCAGGGGCT GCAGGTAGCG CTGTGCAGCC AGGCCAAGCT GCAGGCCCAG
1141     CAGGAGTTGC TGCAGACCAA GCTGGAGCAC CTGGGCCCCG GCGAGCCCCC GCCTGTGCTG
1201     CTCCTGCAGG ATGACCGCCA CTCCACGTCG TCCTCGGAGC AGGAGCGAGA GGGGGGAAGG
1261     ACACCCACGC TGGAGATCCT TAAGAGCCAC ATCTCAGGAA TCTTCCGCCC CAAGTTCTCG
1321     CTCCCTCCAC CGCTGCAGCT CATTCCGGAG GTGCAGAAGC CCCTGCATGA GCAGCTGTGG
1381     TACCACGGGG CCATCCCGAG GGCAGAGGTG GCTGAGCTGC TGGTGCACTC TGGGGACTTC
1441     CTGGTGCGGG AGAGCCAGGG CAAGCAGGAG TACGTGCTGT CGGTGCTGTG GGATGGTCTG
1501     CCCCGGCACT TCATCATCCA GTCCTTGGAT AACCTGTACC GACTGGAAGG GGAAGGCTTT
1561     CCTAGCATTC CTTTGCTCAT CGACCACCTA CTGAGCACCC AGCAGCCCCT CACCAAGAAG
1621     AGTGGTGTTG TCCTGCACAG GGCTGTGCCC AAGGACAAGT GGGTGCTGAA CCATGAGGAC
1681     CTGGTGTTGG GTGAGCAGAT TGGACGGGGG AACTTTGGCG AAGTGTTCAG CGGACGCCTG
1741     CGAGCCGACA ACACCCTGGT GGCGGTGAAG TCTTGTCGAG AGACGCTCCC ACCTGACCTC
1801     AAGGCCAAGT TTCTACAGGA AGCGAGGATC CTGAAGCAGT ACAGCCACCC CAACATCGTG
1861     CGTCTCATTG GTGTCTGCAC CCAGAAGCAG CCCATCTACA TCGTCATGGA GCTTGTGCAG
1921     GGGGGCGACT TCCTGACCTT CCTCCGCACG GAGGGGGCCC GCCTGCGGGT GAAGACTCTG
1981     CTGCAGATGG TGGGGGATGC AGCTGCTGGC ATGGAGTACC TGGAGAGCAA GTGCTGCATC
2041     CACCGGGACC TGGCTGCTCG GAACTGCCTG GTGACAGAGA AGAATGTCCT GAAGATCAGT
2101     GACTTTGGGA TGTCCCGAGA GGAAGCCGAT GGGGTCTATG CAGCCTCAGG GGGCCTCAGA
2161     CAAGTCCCCG TGAAGTGGAC CGCACCTGAG GCCCTTAACT ACGGCCGCTA CTCCTCCGAA
2221     AGCGACGTGT GGAGCTTTGG CATCTTGCTC TGGGAGACCT TCAGCCTGGG GGCCTCCCCC
2281     TATCCCAACC TCAGCAATCA GCAGACACGG GAGTTTGTGG AGAAGGGGGG CCGTCTGCCC
2341     TGCCCAGAGC TGTGTCCTGA TGCCGTGTTC AGGCTCATGG AGCAGTGCTG GGCCTATGAG
2401     CCTGGGCAGC GGCCCAGCTT CAGCACCATC TACCAGGAGC TGCAGAGCAT CCGAAAGCGG
2461     CATCGGTGA

 

Quality Control:cDNA sequencing results is available upon request.