Human FCRL1 Gene cDNA Clone / ORF Clone

FCRL1 cDNA Clone Datasheet

FCRL1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_052938.4
• cDNA Size: 1290
• Gene Synonym: FCRH1; IFGP1; IRTA5; DKFZp667O1421; FCRL1
• Source: Human

FCRL1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens chemokine (C-X-C motif) receptor 7 DNA.
• Vector: pMD18-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 711G/A not causing the amino acid variation.

FCRL1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FCRL1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

FCRL1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTGCCGA GGCTGTTGCT GTTGATCTGT GCTCCACTCT GTGAACCTGC CGAGCTGTTT
61       TTGATAGCCA GCCCCTCCCA TCCCACAGAG GGGAGCCCAG TGACCCTGAC GTGTAAGATG
121      CCCTTTCTAC AGAGTTCAGA TGCCCAGTTC CAGTTCTGCT TTTTCAGAGA CACCCGGGCC
181      TTGGGCCCAG GCTGGAGCAG CTCCCCCAAG CTCCAGATCG CTGCCATGTG GAAAGAAGAC
241      ACAGGGTCAT ACTGGTGCGA GGCACAGACA ATGGCGTCCA AAGTCTTGAG GAGCAGGAGA
301      TCCCAGATAA ATGTGCACAG GGTCCCTGTC GCTGATGTGA GCTTGGAGAC TCAGCCCCCA
361      GGAGGACAGG TGATGGAGGG AGACAGGCTG GTCCTCATCT GCTCAGTTGC TATGGGCACA
421      GGAGACATCA CCTTCCTTTG GTACAAAGGG GCTGTAGGTT TAAACCTTCA GTCAAAGACC
481      CAGCGTTCAC TGACAGCAGA GTATGAGATT CCTTCAGTGA GGGAGAGTGA TGCTGAGCAA
541      TATTACTGTG TAGCTGAAAA TGGCTATGGT CCCAGCCCCA GTGGGCTGGT GAGCATCACT
601      GTCAGAATCC CGGTGTCTCG CCCAATCCTC ATGCTCAGGG CTCCCAGGGC CCAGGCTGCA
661      GTGGAGGATG TGCTGGAGCT TCACTGTGAG GCCCTGAGAG GCTCTCCTCC AATCCTGTAC
721      TGGTTTTATC ACGAGGATAT CACCCTGGGG AGCAGGTCGG CCCCCTCTGG AGGAGGAGCC
781      TCCTTCAACC TTTCCCTGAC TGAAGAACAT TCTGGAAACT ACTCCTGTGA GGCCAACAAT
841      GGCCTGGGGG CCCAGCGCAG TGAGGCGGTG ACACTCAACT TCACAGTGCC TACTGGGGCC
901      AGAAGCAATC ATCTTACCTC AGGAGTCATT GAGGGGCTGC TCAGCACCCT TGGTCCAGCC
961      ACCGTGGCCT TATTATTTTG CTACGGCCTC AAAAGAAAAA TAGGAAGACG TTCAGCCAGG
1021     GATCCACTCA GGAGCCTTCC CAGCCCTCTA CCCCAAGAGT TCACCTACCT CAACTCACCT
1081     ACCCCAGGGC AGCTACAGCC TATATATGAA AATGTGAATG TTGTAAGTGG GGATGAGGTT
1141     TATTCACTGG CGTACTATAA CCAGCCGGAG CAGGAATCAG TAGCAGCAGA AACCCTGGGG
1201     ACACATATGG AGGACAAGGT TTCCTTAGAC ATCTATTCCA GGCTGAGGAA AGCAAACATT
1261     ACAGATGTGG ACTATGAAGA TGCTATGTAA

 

Quality Control:cDNA sequencing results is available upon request.