Mouse FCRL1 Gene cDNA Clone / ORF Clone

FCRL1 cDNA Clone Datasheet

FCRL1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001136236.1
• cDNA Size: 903
• Gene Synonym: Bxmh1; Fcrh1; Ifgp1; Bxmas1; Bxmh1b; Fcrh1l; Fcrh1s; A230020G22Rik; Fcrl1
• Source: Mouse

FCRL1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Fc receptor-like 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for four point mutations:304 C/G resulting in the amino acid Pro substitution by Ala, 682 C/T resulting in the amino acid Pro substitution by Ser, 621 A/G and 672 C/G not causing the amino acid variation.

FCRL1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FCRL1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FCRL1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTACCTT GGCTCCTGCT ACTGATCTGT GCTCTACCGT GTGAACCTGC TGGAATCTCT
61       GATGTGAGCT TGAAGACACG GCCCCCAGGA GGATGGGTGA TGGAGGGAGA CAAGCTGGTC
121      CTCATCTGCT CGGTTGATAG AGTCACTGGG AATATAACTT ACTTCTGGTA CAGAGGGGCC
181      CTGGGTTTCC AACTGGAAAC AAAGACACAA CCTTCACTAA CAGCAGAGTT TGAGATCAGT
241      GACATGAAGC AGAGCGATGC TGATCAATAT TACTGTGCGG CTAACGATGG CCACGACCCT
301      ATCGCCAGTG AGCTGGTGAG CATCCACGTC AGAGTTCCAG TGTCTCGCCC TGTCCTTACG
361      TTTGGGGACT CTGGAACCCA GGCTGTGCTA GGGGACCTGG TGGAGCTTCA CTGTAAGGCC
421      CTGAGAGGCT CACCCCCAAT CTTCTACCAG TTTTATCATG AGAGCATCAT CCTGGGGAAC
481      AGTTCAGCAC CCTCTGGAGG AGGAGCATCC TTCAACTTCT CCCTGACTGC AGAACATTCT
541      GGAAACTTCT CCTGTGAGGC CAGCAATGGA CAGGGTGCCC AACGAAGTGA GGTGGTGGCT
601      CTCAACTTAA CAGGAAGACA GTCAGAGGAT CCAGTCAGGA GCCCTCCTCA GACTGTGCTC
661      CAAGGATCCA CGTACCCCAA ATCCCCCGAC TCAAGGCAGC CAGAGCCCCT GTATGAGAAC
721      GTGAACGTTG TAAGTGGCAA TGAAGTGTAC TCTCTGGTGT ACCACACCCC GCAGGTGCTG
781      GAACCAGCAG CAGCTCAGCA TGTGAGGACA CACGGAGTAA GTGAGTCCTT TCAGGTCTCC
841      TCTGGACTCT ATTCTAAGCC AAGGATAAAC ATTGCACATA TGGACTATGA AGACGCCATG
901      TAG

 

Quality Control:cDNA sequencing results is available upon request.