Rat FCNB Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80252-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

FCNB cDNA Clone Datasheet

FCNB cDNA Clone Product Information

Gene Bank Ref.ID:	NM_053634.1
cDNA Size:	960
Gene Synonym:	Fcn2;Fcnb
Source:	Rat

FCNB cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus ficolin B DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

FCNB cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FCNB cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FCNB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTCCTGG GATCTGCTGC ACTATTCGTC TTGAGCCTGT GTGTCACTGA GCTGACCCTC
61       CATGCTGCTG ACACCTGCCC AGAGGTGAAG GTCCTGGATC TGGAGGGCTC CAACAAACTC
121      ACCATCCTTC AAGGCTGCCC TGGCTTGCCT GGAGCCCTGG GGCCCAAGGG AGAGGCTGGT
181      GCCAAAGGAG ATAGAGGAGA GAGTGGCCTT CCTGGACACC CTGGAAAGGC GGGGCCAACT
241      GGACCCAAAG GAGACCGAGG AGAGAAGGGA GTGCGTGGAG AAAAAGGAGA CACCGGGCCC
301      TCCCAGTCAT GTGCTACAGG ACCTCGGACC TGCAAGGAGT TGCTCACCCG GGGCTACTTT
361      CTCACTGGCT GGTATACCAT CTACCTGCCA GACTGCAGGC CCCTGACCGT GCTGTGTGAT
421      ATGGACACAG ATGGTGGAGG CTGGACCGTC TTTCAGAGAA GGATCGACGG CACGGTGGAC
481      TTCTTTCGGG ACTGGACCTC ATACAAGCAA GGCTTCGGCA GCCAGCTGGG GGAGTTCTGG
541      CTGGGGAACG ATAACATCCA TGCTTTAACC ACCCAGGGAA CCAATGAGCT GCGGGTGGAT
601      CTTGCAGACT TCGACGGCAA CCATGACTTT GCCAAGTACA GCTCCTTCCA GATCCAGGGA
661      GAGGCCGAGA AATACAAGCT CATTCTGGGG AACTTCCTCG GTGGTGGTGC TGGTGACTCC
721      CTGACTTCCC AAAACAACAT GCTTTTCTCC ACCAAAGACC AAGACAACGA CCAGGGATCC
781      TCCAACTGTG CCGTGCGTTA CCATGGAGCC TGGTGGTACT CTGATTGTCA CACTTCCAAC
841      CTGAATGGCC TCTACCTTAG GGGTCTCCAT AAGAGCTATG CAAACGGTGT CAATTGGAAG
901      TCATGGAAAG GGTACAACTA CAGCTACAAG GTTTCTGAAA TGAAGGTGCG CCTCATCTAG

Quality Control:cDNA sequencing results is available upon request.