Cynomolgus monkey FCN1 Gene cDNA Clone / ORF Clone

FCN1 cDNA Clone Datasheet

FCN1 cDNA Clone Product Information

• cDNA Size: 981
• Gene Synonym: FCN1
• Source: Cynomolgus monkey

FCN1 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) ficolin (collagen/fibrinogen domain containing) 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 171 C/T，732 G/A，957 A/C not causing the amino acid variation.
  Please check the sequence information before order.

FCN1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FCN1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FCN1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGCTGA GTGGAGCCAC CATGGCCCGG GGGCTCGCTG TCCTACTAGT CTTGTTCCTG
61       CATATCAAGG ACCTGCCTGC CCAGGCTGCT GACACCTGTC CAGAGGTGAA GGTGGTGGGC
121      CTGGAGGGCT CTGACAAGCT CACCATTCTC CGAGGCTGCC CGGGGCTGCC TGGGGCCCCA
181      GGACCAAAGG GAGAGGCAGG CATCAATGGA AAGACAGGAG AACGCGGTAT CCCTGGAGCC
241      CCTGGAAAGG TGGGACCAGT GGGACCCAAA GGAGACCGAG GAGAGAAGGG GATGCGTGGA
301      GAGAAAGGAG ATGCTGGGCA GTCTCAGTCT TGTGCGACAG GTCCACGCAC CTGCAAGGAC
361      CTACTAGACC GAGGGCACTT CCTGAGTGGC TGGTACACCA TCTACCTGAC CAACTGCCGG
421      CTGCTGGCTG TGCTCTGTGA CATGGACACG GACGGAGGGG GCTGGACCGT TTTCCAGCGG
481      AGGATGGACG GCTCCGTGGA CTTCTACCGG GACTGGGCCG CGTACAAGCA GGGCTTTGGC
541      AGTCGGCTGG GGGAGTTCTG GCTGGGGAAC GACAACATCC ACGCCCTGAC CGCCCAGGGA
601      AGCAGCGAGC TCCGTGTAGA CCTGGTGGAC TTTGAGGGCA ATCGCCAGTT TGCTAAGTAT
661      AGATCATTCA AGGTGGCCGG TGAGGCGGAG AAGTACAAGC TAGTACTGGG AGCCTTTGTT
721      GAGGGCAGTG CAGGTAATTC TCTAGGGGCC CACAACAACC ACTTCTTCTC CACCAAAGAC
781      CAAGACAACG ATGTGAGTTC TTCGAACTGT GCTGTGAAGT TCCAGGGAGC CTGGTGGTAC
841      TCCGACTGTC ACGCTTCAAA CCTGAATGGT CTCTACCTCA GAGGACCCCA TGAGAGCTAT
901      GCCAACGGCA TCAACTGGAG TGCGGCAAAG GGGTACAACT ATAGCTACAA GGTGTCCGAG
961      ATGAAGATGC GGCCCGCCTA G

 

Quality Control:cDNA sequencing results is available upon request.