Mouse FCGRT Gene cDNA Clone / ORF Clone

FCGRT cDNA Clone Datasheet

FCGRT cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010189.3
• cDNA Size: 1098
• Gene Synonym: FcRn; Fcgrt
• Source: Mouse

FCGRT cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Fc receptor, IgG, alpha chain transporter DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for two point mutations: 141 T/C and 958 T/C not causing the amino acid variation.

FCGRT cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FCGRT cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FCGRT cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGATGC CACTGCCCTG GGCCCTCAGC CTCTTGTTGG TCCTCCTGCC TCAGACCTGG
61       GGCTCAGAGA CCCGCCCCCC ACTGATGTAT CATCTCACGG CTGTGTCAAA CCCATCTACG
121      GGGCTTCCCT CTTTCTGGGC CACAGGCTGG TTGGGTCCTC AGCAGTATCT GACCTACAAC
181      AGCCTGCGGC AGGAAGCTGA CCCCTGTGGG GCCTGGATGT GGGAAAATCA GGTGTCTTGG
241      TATTGGGAGA AGGAGACCAC AGACCTCAAA AGCAAAGAAC AGCTCTTCTT GGAGGCCCTC
301      AAGACCCTGG AGAAGATATT AAATGGGACC TACACACTGC AGGGCCTGCT GGGCTGTGAA
361      CTGGCCTCGG ATAATTCCTC AGTGCCCACG GCTGTGTTTG CCCTCAATGG TGAGGAGTTT
421      ATGAAATTCA ACCCAAGAAT CGGCAATTGG ACTGGGGAGT GGCCTGAGAC GGAAATCGTT
481      GCTAATCTGT GGATGAAGCA GCCTGATGCG GCCAGGAAGG AGAGCGAGTT CCTGCTAAAC
541      TCTTGTCCGG AGCGACTGCT AGGCCACCTG GAGAGGGGCC GACGGAACCT GGAGTGGAAG
601      GAGCCGCCGT CTATGCGCCT GAAGGCCCGT CCTGGCAACT CTGGCTCCTC CGTGCTGACC
661      TGTGCTGCTT TCTCCTTCTA CCCACCGGAG CTCAAGTTCC GATTCCTGCG CAATGGGCTA
721      GCCTCAGGCT CCGGGAATTG CAGCACTGGT CCCAATGGAG ATGGCTCTTT CCACGCATGG
781      TCATTGCTGG AGGTCAAACG TGGAGATGAG CACCATTATC AATGTCAAGT GGAGCATGAG
841      GGGCTGGCAC AGCCTCTCAC TGTGGACCTA GATTCATCAG CCAGATCTTC TGTGCCTGTG
901      GTTGGAATCG TTCTTGGTTT ATTGCTGGTG GTAGTGGCCA TCGCAGGCGG TGTGCTGCTG
961      TGGGGCAGGA TGCGCAGCGG TCTGCCAGCC CCATGGCTTT CTCTCAGCGG CGATGACTCT
1021     GGTGACCTGT TGCCTGGTGG GAACTTGCCC CCAGAAGCTG AACCTCAAGG TGCAAATGCC
1081     TTTCCAGCCA CTTCCTGA

 

Quality Control:cDNA sequencing results is available upon request.