CD32B cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG10260-M-F
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CD32B cDNA ORF Clone, Human, C-DDK (Flag®) tag General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
873 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human Fc fragment of IgG, low affinity IIb, receptor (FCGR2B), transcript variant 2 DNA.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV/hygro-FLAG
Restriction Sites
KpnI + XbaI
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
CD32B cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CD32B cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images
Human CD32b/FCGR2B/Fc gamma RIIB Gene Expression validated Image 2396
The plasmid was transfected into 293E adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope.
Human Vimentin Gene Plasmid Map 7163
CD32B cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
CD32 cDNA ORF Clone, Human;CD32B cDNA ORF Clone, Human;Fc gamma RIIB cDNA ORF Clone, Human;FCGR2 cDNA ORF Clone, Human
CD32B Background Information
Full Name
Fc fragment of IgG, low affinity IIb, receptor (CD32)

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