Human FCER1A Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13193-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

FCER1A cDNA Clone Datasheet

FCER1A cDNA Clone Product Information

Gene Bank Ref.ID:	NM_002001.2
cDNA Size:	774
Gene Synonym:	FCE1A; FcERI; FCER1A
Source:	Human

FCER1A cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

FCER1A cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FCER1A cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FCER1A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCCTG CCATGGAATC CCCTACTCTA CTGTGTGTAG CCTTACTGTT CTTCGCTCCA
61       GATGGCGTGT TAGCAGTCCC TCAGAAACCT AAGGTCTCCT TGAACCCTCC ATGGAATAGA
121      ATATTTAAAG GAGAGAATGT GACTCTTACA TGTAATGGGA ACAATTTCTT TGAAGTCAGT
181      TCCACCAAAT GGTTCCACAA TGGCAGCCTT TCAGAAGAGA CAAATTCAAG TTTGAATATT
241      GTGAATGCCA AATTTGAAGA CAGTGGAGAA TACAAATGTC AGCACCAACA AGTTAATGAG
301      AGTGAACCTG TGTACCTGGA AGTCTTCAGT GACTGGCTGC TCCTTCAGGC CTCTGCTGAG
361      GTGGTGATGG AGGGCCAGCC CCTCTTCCTC AGGTGCCATG GTTGGAGGAA CTGGGATGTG
421      TACAAGGTGA TCTATTATAA GGATGGTGAA GCTCTCAAGT ACTGGTATGA GAACCACAAC
481      ATCTCCATTA CAAATGCCAC AGTTGAAGAC AGTGGAACCT ACTACTGTAC GGGCAAAGTG
541      TGGCAGCTGG ACTATGAGTC TGAGCCCCTC AACATTACTG TAATAAAAGC TCCGCGTGAG
601      AAGTACTGGC TACAATTTTT TATCCCATTG TTGGTGGTGA TTCTGTTTGC TGTGGACACA
661      GGATTATTTA TCTCAACTCA GCAGCAGGTC ACATTTCTCT TGAAGATTAA GAGAACCAGG
721      AAAGGCTTCA GACTTCTGAA CCCACATCCT AAGCCAAACC CCAAAAACAA CTGA

Quality Control:cDNA sequencing results is available upon request.