|Datasheet||Specific References||Reviews||Related Products||Protocols|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human FCER1A Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG13193-G-F|
|Human FCER1A Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG13193-G-H|
|Human FCER1A Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG13193-G-M|
|Human FCER1A Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG13193-G-N|
|Human FCER1A Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG13193-G-Y|
FcERI, also known as FCER1A, is the alpha subunit of the immunoglobulin epsilon receptor (IgE receptor). IgE receptor is a high affinity IgE receptor which plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. IgE receptor is comprised of an alpha subunit(FcERI), a beta subunit, and two gamma subunits. FcERI is glycosylated and contains 2 Ig-like (immunoglobulin-like) domains.