Human FASLG Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13885-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

FASLG cDNA Clone Datasheet

FASLG cDNA Clone Product Information

Gene Bank Ref.ID:	BC017502
cDNA Size:	846
Gene Synonym:	APT1LG1, CD178, CD95-L, CD95L, FASL, TNFSF6,FASLG
Source:	Human

FASLG cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens Fas ligand (TNF superfamily, member 6) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

FASLG cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

FASLG cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

FASLG cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAGCAGC CCTTCAATTA CCCATATCCC CAGATCTACT GGGTGGACAG CAGTGCCAGC
61       TCTCCCTGGG CCCCTCCAGG CACAGTTCTT CCCTGTCCAA CCTCTGTGCC CAGAAGGCCT
121      GGTCAAAGGA GGCCACCACC ACCACCGCCA CCGCCACCAC TACCACCTCC GCCGCCGCCG
181      CCACCACTGC CTCCACTACC GCTGCCACCC CTGAAGAAGA GAGGGAACCA CAGCACAGGC
241      CTGTGTCTCC TTGTGATGTT TTTCATGGTT CTGGTTGCCT TGGTAGGATT GGGCCTGGGG
301      ATGTTTCAGC TCTTCCACCT ACAGAAGGAG CTGGCAGAAC TCCGAGAGTC TACCAGCCAG
361      ATGCACACAG CATCATCTTT GGAGAAGCAA ATAGGCCACC CCAGTCCACC CCCTGAAAAA
421      AAGGAGCTGA GGAAAGTGGC CCATTTAACA GGCAAGTCCA ACTCAAGGTC CATGCCTCTG
481      GAATGGGAAG ACACCTATGG AATTGTCCTG CTTTCTGGAG TGAAGTATAA GAAGGGTGGC
541      CTTGTGATCA ATGAAACTGG GCTGTACTTT GTATATTCCA AAGTATACTT CCGGGGTCAA
601      TCTTGCAACA ACCTGCCCCT GAGCCACAAG GTCTACATGA GGAACTCTAA GTATCCCCAG
661      GATCTGGTGA TGATGGAGGG GAAGATGATG AGCTACTGCA CTACTGGGCA GATGTGGGCC
721      CGCAGCAGCT ACCTGGGGGC AGTGTTCAAT CTTACCAGTG CTGATCATTT ATATGTCAAC
781      GTATCTGAGC TCTCTCTGGT CAATTTTGAG GAATCTCAGA CGTTTTTCGG CTTATATAAG
841      CTCTAA

Quality Control:cDNA sequencing results is available upon request.