FANCA / FACA Protein (His Tag)

Fanconi anemia, complementation group A Protein Datasheet

FANCA / FACA Protein Product Information

• Synonym: FA, FA-H, FA1, FAA, FACA, FAH, FANCH, MGC75158
• Protein Construction: A DNA sequence encoding the human FANCA (AAH08979.1) (Met 1-Cys 297) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

FANCA / FACA Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human FANCA consists of 316 amino acids and predicts a molecular mass of 35.2 kDa. It migrates as an approximately 35 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 200mM NaCl, pH 8.5, 20%gly, 0.1% Triton, 1mM TCEP
• SDS-PAGE: FANCA / FACA protein

FANCA / FACA Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FANCA / FACA Protein Description

FANCA is one of the six known Fanconi anemia gene products (FANCA, FANCC, FANCD2, FANCE, FANCF, and FANCG proteins). Fanconi anemia (FA) is a genetic disorder predisposing to aplastic anemia and cancer characterized by hypersensitivity to DNA-damaging agents and oxidative stress. These FA proteins don't share sequence similarity; they are related by their assembly into a nucleu protein complex. They appear to be involved in a common functional pathway that is required for the monoubiquitination of a sixth gene product,k FANCD2. FANCA associates with the IκB kinase (IKK) signalsome via interaction with IKK2. Components of the FANCA complex undergo rapid, stimulus-dependent changes in phosphorylation, which are blocked by kinase-inactive IKK2 (IKK2 K > M). When exposed to mitomycin C, cells expressing IKK2 K > M develop a cell cycle abnormality characteristic of FA. Thus, FANCA may function to recruit IKK2, thus providing the cell a means of rapidly responding to stress.

References

1. Otsuki T. et al., 2002, J Cell Biochem. 86 (4): 613-23.
2. Taniguchi T. et al., 2002, Blood. 100 (7): 2457-62.
3. Garcia-Higuera I. et al., 1999, Mol Cell Biol. 19 (7): 4866-73.