Mouse FAM3D / Oit1 Protein (His Tag) (Cytokine)

Oncoprotein induced transcript 1 Protein Datasheet

FAM3D / Oit1 Protein Product Information

• Synonym: FAM3D, Oit1
• Protein Construction: A DNA sequence encoding the mouse FAM3D (P97805) (Met1-Met223) was expressed with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

FAM3D / Oit1 Protein QC Testing

• Purity: > 90% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Thr 26
• Molecular Mass: The recombinant mouse FAM3D comprises 209 amino acids and has a predicted molecular mass of 23.5 kDa. The apparent molecular mass of the protein is approximately 23 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FAM3D / Oit1 protein

FAM3D / Oit1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FAM3D / Oit1 Protein Description

Family with sequence similarity 3 (FAM3) family is a novel cytokine-like family. There are four genes in this family, FAM3A, FAM3B, FAM3C, and FAM3D, each encoding a protein (224-235 amino acids) with a hydrophobic leader sequence. It had indicated that FAM3B/PANDER (pancreatic derived factor) is highly expressed in pancreas, and FAM3A and FAM3C in almost all tissues. FAM3D is abundantly expressed in placenta and weakly expressed in small intestine. Immunohistochemistry showed that FAM3A is expressed prominently in the vascular endothelium, particularly capillaries. FAM3A and FAM3B protein were both localized to the islets of Langerhans of the endocrine pancreas. Recombinant FAM3B protein has delayed effects on beta-cell function, inhibiting basal insulin secretion from a beta-cell line in a dose-dependent manner. FAM3C is involved in retinal laminar formation processes in vertebrates. NFATC2, SCP2, CACNA1C, TCRA, POLE, and FAM3D, were associated with narcolepsy. Some of these associations were further supported by gene expression analyses and an association study in essential hypersomnia (EHS), CNS hypersonia similar to narcolepsy.

References

1. Zhu Y. et al., 2002, Genomics. 80(2): 144-50.
2. Katahira T. et al.,2010, Biochem Biophys Res Commun. 392 (3): 301-6.
3. Shimada M. et al., 2010, Hum Genet. 128(4): 433-41.