FABP4 / ALBP / A-FABP Protein (His Tag)

Fatty Acid-Binding Protein 4 ( FABP4 / ALBP / A-FABP ) Protein

FABP4 / ALBP / A-FABP Protein Product Information

• Synonym: A-FABP, aP2
• Protein Construction: A DNA sequence encoding the human FABP4 ( P15090 ) ( Cys 2 - Ala 132 ) ( 29 Ala / Thr ) was expressed, with a polyhistide tag at the N-terminus
• Source: Human
• Expression Host: E. coli

FABP4 / ALBP / A-FABP Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human FABP4 consisting of 142 amino acids and has a calculated molecular mass of 16 kDa. It migrates as an approximately 15 kDa band in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile 50mM Tris, 200mM NaCl, 10% glycerol, pH 8.2
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FABP4 protein

FABP4 / ALBP / A-FABP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

FABP4 / ALBP / A-FABP Protein Description

Fatty acid-binding protein, adipocyte, also known as Adipocyte-type fatty acid-binding protein, Fatty acid-binding protein 4, Adipocyte lipid-binding protein, and FABP4, is a cytoplasm protein which belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. In familial combined hyperlipidemia (FCHL), FABP4 correlated to body mass index (BMI), waist circumference, insulin levels, and homeostasis model assessment (HOMA) index.FABP4 levels were associated with triglyceride-rich lipoproteins independent of insulin resistance. FABP4 is an adipokine regulating systemic insulin sensitivity, lipid and glucose metabolism. In humans serum FABP4 levels correlate significantly with features of PCOS. It appears to be a determinant of atherogenic dyslipidemia. FABP4 pathway mediates the sebaceous gland hyperplasia in keratinocyte-specific Pten-null mice. FABP4 concentration significantly increased with an increasing of MS features and was strongly correlated with body-mass index, triglycerides, HDL-cholesterol concentrations, insulin and blood pressure. Patients in the highest quartile of FABP4 presented a six-fold increased odds ratio for MS and a three-fold increased odds for LD, adjusted by age, sex, body-mass index and the antiretroviral therapy. FABP4 is a strong plasma marker of metabolic disturbances in HIV-infected patients, and therefore, could serve to guide therapeutic intervention in this group of patients.

References

1. van Dongen,M.J. et al., 2002, J Am Chem Soc. 124 (40): 11874-80.
2. Coll, B. et al., 2008, Atherosclerosis  199 (1):147-53.
3. Hoashi,S. et al., 2008, BMC Genet. 9 :84.
4. Cai,H. et al., 2010, Bioorg Med Chem Lett  20 (12):3675-9.