FABP4 / ALBP Antibody (Antigen Affinity Purified)

Mouse Fatty Acid-binding Protein Antibody ( Antigen Affinity Purified )

FABP4 / ALBP Antibody Product Information

• Immunogen: Recombinant mouse FABP4 protein ( Catalog#50652-M08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant mouse FABP4 ( rM FABP4 ; Catalog#50652-M08H ; NP_077717.1 ; Met 1 - Ala 132 ). FABP4 specific IgG was purified by mouse FABP4 affinity chromatography

FABP4 / ALBP Antibody Usage Guide

• Specificity: Mouse FABP4 / ALBP
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect mouse FABP4. The detection limit for mouse FABP4 is approximately 0.0195 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

FABP4 / ALBP Antibody Background

Mouse fatty acid-binding protein, adipocyte, also known as Adipocyte-type fatty acid-binding protein, Fatty acid-binding protein 4, Adipocyte lipid-binding protein, and FABP4, is a cytoplasm protein which belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. In familial combined hyperlipidemia (FCHL), FABP4 correlated to body mass index (BMI), waist circumference, insulin levels, and homeostasis model assessment (HOMA) index.FABP4 levels were associated with triglyceride-rich lipoproteins independent of insulin resistance. FABP4 is an adipokine regulating systemic insulin sensitivity, lipid and glucose metabolism. In humans serum FABP4 levels correlate significantly with features of PCOS. It appears to be a determinant of atherogenic dyslipidemia. FABP4 pathway mediates the sebaceous gland hyperplasia in keratinocyte-specific Pten-null mice. FABP4 concentration significantly increased with an increasing of MS features and was strongly correlated with body-mass index, triglycerides, HDL-cholesterol concentrations, insulin and blood pressure. Patients in the highest quartile of FABP4 presented a six-fold increased odds ratio for MS and a three-fold increased odds for LD, adjusted by age, sex, body-mass index and the antiretroviral therapy. FABP4 is a strong plasma marker of metabolic disturbances in HIV-infected patients, and therefore, could serve to guide therapeutic intervention in this group of patients.

References

1. van Dongen,M.J. et al., 2002, J Am Chem Soc. 124 (40): 11874-80.
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3. Hoashi,S. et al., 2008, BMC Genet. 9 :84.
4. Cai,H. et al., 2010, Bioorg Med Chem Lett  20 (12):3675-9.