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Rat F3 Gene cDNA Clone (full-length ORF Clone)
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Rat F3 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
RG80407-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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F3 cDNA Clone Datasheet

 

F3 cDNA Clone Product Information

Gene Bank Ref.ID: NM_013057.2

cDNA Size:

888

Gene Synonym:

MGC93621; F3

Source: Rat

F3 cDNA Clone Description

cDNA Description:

ORF Clone of Rattus norvegicus coagulation factor III (thromboplastin, tissue factor) DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 414 C/T not causing the amino acid variation.

F3 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

F3 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

F3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTATCC CCATGCGCCC GCGCCTCCTA GCGGCCCTCG CGCCCACCTT TCTCGGCTTC
61       CTTCTCCTTC AGGTGGCCGC TGGTGCAGGC ACTCCTCCAG GGAAAGCGTT TAATTTAACT
121      TGGATATCAA CTGATTTCAA GACAATCTTG GAGTGGCAAC CGAAACCCAC CAACTATACC
181      TACACTGTTC AGATAAGCGA TAGATCTAGA AACTGGAAAT ACAAATGCAC TGGAACCACA
241      GACACTGAGT GTGACCTCAC CGACGAGATT GTGAAGGATG TGAACTGGAC CTATGAAGCA
301      AGGGTCCTAT CTGTCCCATG GAGGAACTCA ACTCATGGAA AGGAAACACT CTTTGGAACT
361      CATGGGGAGG AACCGCCATT TACAAATGCC CGGAAGTTTT TACCTTACCG AGATACAAAA
421      ATTGGACAGC CAGTAATTCA GAAGTATGAA CAAGACGGTA CGAAACTGAA GGTGACTGTA
481      AAAGACTCAT TTACATTAGT CAGAAAGAAT GGTACATTCC TCACTCTGCG GCAAGTTTTT
541      GGCAATGACT TGGGTTATAT TCTTACGTAT CGGAAAGACT CAAGCACAGG AAGGAAAACA
601      AACACTACAC ATACCAATGA ATTCTTGATT GATGTGGAAA AGGGGGTAAG CTACTGCTTC
661      TTCGTACAAG CCGTGATTTT CTCCAGGAAA ACTAACCACA AGAGCCCAGA AAGCATCACC
721      AAGTGCACTG AGCAATGGAA GAGTGTCCTG GGAGAAACAC TCATCATTGT GGGAGCAGTG
781      GTGTTCCTGG TCACTGTCTT TATCATCCTG CTGACCATAT CTCTGTGCAA GCGCAGAAAG
841      AACAGAGCAG GACAGAAAAG GAAGAACACC CCGTCGCGCT TGGCATAG

 

Quality Control:cDNA sequencing results is available upon request.

 

 

Tissue Factor (Coagulation Factor III, CD142) related areas, pathways, and other information

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"