Human F3 Gene cDNA Clone / ORF Clone

Human F3 cDNA Clone Datasheet

F3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001993.4
• cDNA Size: 888
• Gene Synonym: CD142, FLJ17960, TF, TFA
• Source: Human

F3 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens coagulation factor III (thromboplastin, tissue factor) DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

F3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

F3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

F3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGACCC CTGCCTGGCC CCGGGTCCCG CGCCCCGAGA CCGCCGTCGC TCGGACGCTC
61       CTGCTCGGCT GGGTCTTCGC CCAGGTGGCC GGCGCTTCAG GCACTACAAA TACTGTGGCA
121      GCATATAATT TAACTTGGAA ATCAACTAAT TTCAAGACAA TTTTGGAGTG GGAACCCAAA
181      CCCGTCAATC AAGTCTACAC TGTTCAAATA AGCACTAAGT CAGGAGATTG GAAAAGCAAA
241      TGCTTTTACA CAACAGACAC AGAGTGTGAC CTCACCGACG AGATTGTGAA GGATGTGAAG
301      CAGACGTACT TGGCACGGGT CTTCTCCTAC CCGGCAGGGA ATGTGGAGAG CACCGGTTCT
361      GCTGGGGAGC CTCTGTATGA GAACTCCCCA GAGTTCACAC CTTACCTGGA GACAAACCTC
421      GGACAGCCAA CAATTCAGAG TTTTGAACAG GTGGGAACAA AAGTGAATGT GACCGTAGAA
481      GATGAACGGA CTTTAGTCAG AAGGAACAAC ACTTTCCTAA GCCTCCGGGA TGTTTTTGGC
541      AAGGACTTAA TTTATACACT TTATTATTGG AAATCTTCAA GTTCAGGAAA GAAAACAGCC
601      AAAACAAACA CTAATGAGTT TTTGATTGAT GTGGATAAAG GAGAAAACTA CTGTTTCAGT
661      GTTCAAGCAG TGATTCCCTC CCGAACAGTT AACCGGAAGA GTACAGACAG CCCGGTAGAG
721      TGTATGGGCC AGGAGAAAGG GGAATTCAGA GAAATATTCT ACATCATTGG AGCTGTGGTA
781      TTTGTGGTCA TCATCCTTGT CATCATCCTG GCTATATCTC TACACAAGTG TAGAAAGGCA
841      GGAGTGGGGC AGAGCTGGAA GGAGAACTCC CCACTGAATG TTTCATAA

 

Quality Control:cDNA sequencing results is available upon request.