Rat F3 / Tissue factor Protein (His Tag)

Coagulation factor III (thromboplastin, tissue factor) Protein Datasheet

F3 / Tissue factor Protein Product Information

• Synonym: F3
• Protein Construction: A DNA sequence encoding the rat F3(Q66HI4)(Met1-Glu252) was expressed, fused with a polyhistidine tag at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

F3 / Tissue factor Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 29
• Molecular Mass: The recombinant rat F3 comprises 235 amino acids and predicts a molecular mass of 27.2 kDa. The apparent molecular mass of the recombinant protein is approximately 47-52 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: F3 / Tissue factor protein

F3 / Tissue factor Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

F3 / Tissue factor Protein Description

Tissue factor, also known as coagulation factor III, F3, and CD142, is a single-pass type I membrane protein which belongs to the tissue factor family. Tissue factor is one of the proteins that participate in hemostatic and inflammatory processes. Activated monocytes present in the liver increase expression of tissue factor, and while accumulating in the organ they can intensify inflammation. Tissue factor is the protein that activates the blood clotting system by binding to, and activating, the plasma serine protease, factor VIIa, following vascular injury. Because of its essential role in hemostasis, Tissue factor plays a role in pathology associated with hemostasis, triggering the coagulation system in many thrombotic diseases and the coagulopathies associated with sepsis and other forms of disseminated intravascular coagulation. Tissue factor is not only the main physiological initiator of normal blood coagulation, but is also important in the natural history of solid malignancies in that it potentiates metastasis and angiogenesis and mediates outside-in signalling. Tissue factor is expressed constitutively by many tissues which are not in contact with blood and by other cells upon injury or activation; the latter include endothelial cells, tissue macrophages, and peripheral blood monocytes.

References

1. Lwaleed BA. et al., 2001, J Pathol. 193 (1): 3-12.
2. Morrissey JH. 2004, Int J Hematol. 79 (2): 103-8.
3. Panasiuk A. et al., 2007, Blood Coagul Fibrinolysis. 18 (8): 739-44.
4. Kinev AV. et al., 2008, Lupus. 17 (10): 952-8.